CV-IIL:nový lektin z lidského podmíněného patogenu Chromobacterium violaceum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00014124" target="_blank" >RIV/00216224:14310/05:00014124 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cv-IIL: new lectin from human oportunistic pathogen Chromobacterium violaceum

Popis výsledku v původním jazyce

Pathogen adhesion to the host cell surface followed by its colonisation, invasion and infectivity is usually mediated by protein-carbohydrate interactions. Pseudomonas aeruginosa, an opportunistic pathogen responsible for numerous nosocomial infections in immunocopromised patients, produces a variety of carbohydrate-binding proteins that could be involved in host recognition and adhesion. One of them, PA-IIL, is a fucose binding lectin that is closely related to the virulence of the bacterium [1]. Searching in databases for proteins displaying sequence similarities to PA-IIL revealed new homologous proteins in other opportunistic pathogens like Chromobacterium violaceum. Human infection by Ch. violaceum is rare but when it occurs, it is associated withvery high mortality rate [2]. The gene cv1741 coding a hypothetical protein similar to PA-IIL, was cloned and the resulting recombinant protein, CV-IIL, has been expressed in E. coli and purified to homogeneity. Binding affinities of the

Název v anglickém jazyce

Cv-IIL: new lectin from human oportunistic pathogen Chromobacterium violaceum

Popis výsledku anglicky

Pathogen adhesion to the host cell surface followed by its colonisation, invasion and infectivity is usually mediated by protein-carbohydrate interactions. Pseudomonas aeruginosa, an opportunistic pathogen responsible for numerous nosocomial infections in immunocopromised patients, produces a variety of carbohydrate-binding proteins that could be involved in host recognition and adhesion. One of them, PA-IIL, is a fucose binding lectin that is closely related to the virulence of the bacterium [1]. Searching in databases for proteins displaying sequence similarities to PA-IIL revealed new homologous proteins in other opportunistic pathogens like Chromobacterium violaceum. Human infection by Ch. violaceum is rare but when it occurs, it is associated withvery high mortality rate [2]. The gene cv1741 coding a hypothetical protein similar to PA-IIL, was cloned and the resulting recombinant protein, CV-IIL, has been expressed in E. coli and purified to homogeneity. Binding affinities of the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Glycoconjugate Journal

ISSN

0282-0080

e-ISSN

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Svazek periodika

22

Číslo periodika v rámci svazku

4/5/6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

2

Strana od-do

286-287

Kód UT WoS článku

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EID výsledku v databázi Scopus

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