NEW MODEL FOR EVALUATION OF ENDOCRINE DISRUPTION OF STEROIDOGENESIS BASED ON ADRENOCORTICAL CELL LINE H295R

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00014605" target="_blank" >RIV/00216224:14310/05:00014605 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

NEW MODEL FOR EVALUATION OF ENDOCRINE DISRUPTION OF STEROIDOGENESIS BASED ON ADRENOCORTICAL CELL LINE H295R

Popis výsledku v původním jazyce

Endocrine disrupting mechanisms of xenobiotics that are released to the environment in large quantities has been very important subject of scientific studies in last years. Main concern was focused on comprehending the process of interaction of xenobiotics with estrogenic receptor which was perceived as the most considerable point of action of endocrine disruptors. As it was shown later, other parts of the steroid signaling pathway are no less important for endocrine disruptors mode of action. Steroidogenesis as a very complex process represents one of them. We used human adrenocorticoid carcinoma cell line H295R which retains ability to synthesize complete set of steroids to develop a model suitable for assessment of the effects of xenobiotics on production of main steroids. Cells were exposed to forskolin (activator of steroidogenesis) and/or tested compound. Levels of main steroids (17-estradiol, testosterone, cortisol, aldosterone, 17-OH-progesterone) were assessed directly in expo

Název v anglickém jazyce

NEW MODEL FOR EVALUATION OF ENDOCRINE DISRUPTION OF STEROIDOGENESIS BASED ON ADRENOCORTICAL CELL LINE H295R

Popis výsledku anglicky

Endocrine disrupting mechanisms of xenobiotics that are released to the environment in large quantities has been very important subject of scientific studies in last years. Main concern was focused on comprehending the process of interaction of xenobiotics with estrogenic receptor which was perceived as the most considerable point of action of endocrine disruptors. As it was shown later, other parts of the steroid signaling pathway are no less important for endocrine disruptors mode of action. Steroidogenesis as a very complex process represents one of them. We used human adrenocorticoid carcinoma cell line H295R which retains ability to synthesize complete set of steroids to develop a model suitable for assessment of the effects of xenobiotics on production of main steroids. Cells were exposed to forskolin (activator of steroidogenesis) and/or tested compound. Levels of main steroids (17-estradiol, testosterone, cortisol, aldosterone, 17-OH-progesterone) were assessed directly in expo

Druh

D - Stať ve sborníku

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

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Projekt

<a href="/cs/project/GP525%2F05%2FP160" target="_blank" >GP525/05/P160: In vitro modely pro studium endokrinní disrupce - účinky tradičních a nových typů persistentních organických polutantů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Chemicals, human and environment

ISBN

9949-13-297-5

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

71

Název nakladatele

ETS

Místo vydání

Tallin

Místo konání akce

Toila, Estonsko

Datum konání akce

20. 10. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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