Aktivace AhR huminovými látkami

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00014613" target="_blank" >RIV/00216224:14310/05:00014613 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Activation of Ah-receptor by humic substances

Popis výsledku v původním jazyce

Humic substances (HS) are ubiquitous in the environment. However, some studies indicate that HS can induce a direct adverse effect on human health and hormone-like effects on fish, amphibians and invertebrates. In the present study we investigated possible biochemical mechanism of HS toxicity using reporter gene in vitro bioassay with H4IIE.luc cells. H4IIE.luc are stably transfected hepatoma cells with a luciferase reporter gene that is activated via cytosolic aryl hydrocarbon receptor (AhR). The mostpotent activator of AhR is 2,3,7,8-tetrachlorodibenzo-p-dioxin, but number of structurally diverse compounds has also been found to activate AhR. Aim of this study was to evaluate the dioxin-like activity of humic substances standards purchased from IHSSand Sigma-Aldrich companies. HS were dissolved in 0.05M NaOH, subsequently, lipophilic compounds (including fragments of HS) were extracted by liquid-liquid extraction with hexane/dichloromethane. Organic extract of HS was further treate

Název v anglickém jazyce

Activation of Ah-receptor by humic substances

Popis výsledku anglicky

Humic substances (HS) are ubiquitous in the environment. However, some studies indicate that HS can induce a direct adverse effect on human health and hormone-like effects on fish, amphibians and invertebrates. In the present study we investigated possible biochemical mechanism of HS toxicity using reporter gene in vitro bioassay with H4IIE.luc cells. H4IIE.luc are stably transfected hepatoma cells with a luciferase reporter gene that is activated via cytosolic aryl hydrocarbon receptor (AhR). The mostpotent activator of AhR is 2,3,7,8-tetrachlorodibenzo-p-dioxin, but number of structurally diverse compounds has also been found to activate AhR. Aim of this study was to evaluate the dioxin-like activity of humic substances standards purchased from IHSSand Sigma-Aldrich companies. HS were dissolved in 0.05M NaOH, subsequently, lipophilic compounds (including fragments of HS) were extracted by liquid-liquid extraction with hexane/dichloromethane. Organic extract of HS was further treate

Druh

D - Stať ve sborníku

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

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Projekt

<a href="/cs/project/GP525%2F05%2FP160" target="_blank" >GP525/05/P160: In vitro modely pro studium endokrinní disrupce - účinky tradičních a nových typů persistentních organických polutantů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Advances and Trends in Ecotoxicology

ISBN

80-210-3799-7

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

115-115

Název nakladatele

MU

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

1. 1. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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