Eliminační voltametrie s lineárním skenem jako nová detekční metoda pro DNA senzory

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00014629" target="_blank" >RIV/00216224:14310/05:00014629 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/05:00023570 RIV/62156489:43210/05:00086475

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Elimination voltammetry with linear scan as a new detection method for DNA sensors

Popis výsledku v původním jazyce

The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs).Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The com

Název v anglickém jazyce

Elimination voltammetry with linear scan as a new detection method for DNA sensors

Popis výsledku anglicky

The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs).Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The com

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

SENSORS

ISSN

1424-8220

e-ISSN

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Svazek periodika

5

Číslo periodika v rámci svazku

6-10

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

17

Strana od-do

448-464

Kód UT WoS článku

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EID výsledku v databázi Scopus

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