Electrochemical DNA Sensors Based on Adsorptive and Elimination Techniques

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00052406" target="_blank" >RIV/00216224:14310/11:00052406 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical DNA Sensors Based on Adsorptive and Elimination Techniques

Popis výsledku v původním jazyce

Electrochemistry-based sensors offer good sensitivity, separation ability, low cost for the detection of ODNs and selected DNA sequences or of muteted genes associated with diseases. Numerous developed approaches, such as the platforms or direct or indirect electrochemistry DNA, specific redox indicator, or mediated charge transport, can be improved by the adsorptive stripping technique in connection with elimination voltammetry with linear scan (AdS EVLS). Here we verify our idea on the electrode systems of homo-and hetero-oligodeoxynuleotides (ODNs) including hairpins. The EVLS was applied to the resolution of reduction signals of adenine (A) and cytosine (C) in short synthetic ODNs. The Ads EVLS results showed that the elimination functions chosen are also useful for the study not only of cathodic but also of anodic processes, especially for the anodic processes of guanine. Due to AdS EVLS or AdTS EVLS we are able to evaluate the ODN structure changes on electrode surfaces.

Název v anglickém jazyce

Electrochemical DNA Sensors Based on Adsorptive and Elimination Techniques

Popis výsledku anglicky

Electrochemistry-based sensors offer good sensitivity, separation ability, low cost for the detection of ODNs and selected DNA sequences or of muteted genes associated with diseases. Numerous developed approaches, such as the platforms or direct or indirect electrochemistry DNA, specific redox indicator, or mediated charge transport, can be improved by the adsorptive stripping technique in connection with elimination voltammetry with linear scan (AdS EVLS). Here we verify our idea on the electrode systems of homo-and hetero-oligodeoxynuleotides (ODNs) including hairpins. The EVLS was applied to the resolution of reduction signals of adenine (A) and cytosine (C) in short synthetic ODNs. The Ads EVLS results showed that the elimination functions chosen are also useful for the study not only of cathodic but also of anodic processes, especially for the anodic processes of guanine. Due to AdS EVLS or AdTS EVLS we are able to evaluate the ODN structure changes on electrode surfaces.

Druh

O - Ostatní výsledky

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06035" target="_blank" >LC06035: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů