Kapilární zónová elektroforéza proteinů a neuroprotektivních peptidů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00025072" target="_blank" >RIV/00216224:14310/05:00025072 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Capillary zone electrophoresis of proteins and neuroprotective peptides

Popis výsledku v původním jazyce

Biocompatibility of inner surface of capillaries in CE is critical for separation and detection of proteins and peptides. The problems come from adsorption of the peptides (proteins) on the surface because biomolecules are sensitive to changes in ionic strength, pH, and/or temperature of the electrolyte, etc. Therefore, modification of the fused silica capillary surface is often necessary. Procedures for capillary coating described in literature are usually complicated and time consuming. We optimized three types of relatively simple coating procedures suitable for protein and peptides separation: a/ polyethyleneimine (PEI), b/ 3-glycidoxypropyltrimethoxysilane (GPTMS), c/ 3-aminopropyltriethoxysilane (APS). Composition, concentration and pH of severalbackground electrolyte were tested, namely citrate, phosphate, aminocaproate and TRIS. The capillary surface was characterized by determination of the electroosmotic flow (EOF). The conditions were optimized by running a standard mixture

Název v anglickém jazyce

Capillary zone electrophoresis of proteins and neuroprotective peptides

Popis výsledku anglicky

Biocompatibility of inner surface of capillaries in CE is critical for separation and detection of proteins and peptides. The problems come from adsorption of the peptides (proteins) on the surface because biomolecules are sensitive to changes in ionic strength, pH, and/or temperature of the electrolyte, etc. Therefore, modification of the fused silica capillary surface is often necessary. Procedures for capillary coating described in literature are usually complicated and time consuming. We optimized three types of relatively simple coating procedures suitable for protein and peptides separation: a/ polyethyleneimine (PEI), b/ 3-glycidoxypropyltrimethoxysilane (GPTMS), c/ 3-aminopropyltriethoxysilane (APS). Composition, concentration and pH of severalbackground electrolyte were tested, namely citrate, phosphate, aminocaproate and TRIS. The capillary surface was characterized by determination of the electroosmotic flow (EOF). The conditions were optimized by running a standard mixture

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA305%2F03%2F1100" target="_blank" >GA305/03/1100: Syntéza a studium neuroprotektivních peptidů odvozených od humaninu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Third International and Twenty-Eighth European Peptide Symposium. Peptides 2004.

ISBN

965-90833-0-0

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

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Název nakladatele

Kenes International

Místo vydání

Geneva, Switzerland

Místo konání akce

Praha

Datum konání akce

1. 1. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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