Role fosforylace při aktivaci a inhibici CDK2 a CDK5 kináz

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00016379" target="_blank" >RIV/00216224:14310/06:00016379 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

THE ROLE OF PHOSPHORYLATION IN CYCLIN DEPENDENT KINASE 2 AND 5 ACTIVATION AND INHIBITION. A COMPUTER SIMULATION STUDY.

Popis výsledku v původním jazyce

We have used molecular dynamics to help understanding different mechanism of CDK2 and CDK5 activation and inhibition. The data were obtained from several simulations including fully active CDK2 in a complex with a short peptide substrate. and simulationson CDK5 in complexes with the protein activator p25 and the purine-like inhibitor roscovitine. Differences between the CDK5/p25 and CDK2/Cyclin A systems are discussed with respect to their specific functionality. We will show why the CDK2 inhibition segment becomes an activation segment for CDK5. We will also clarify why only one step is necessary to activate CDK5 (interaction with the regulatory subunit) while CDK2 needs a two-step activation (other phosphorylation in the activation loop). As a ?sideeffect? information obtained from simulations, we will show how a detailed analysis of the interactions between the protein and the solvent could be used to design new inhibitors. This is especially important from the pharmacological poi

Název v anglickém jazyce

THE ROLE OF PHOSPHORYLATION IN CYCLIN DEPENDENT KINASE 2 AND 5 ACTIVATION AND INHIBITION. A COMPUTER SIMULATION STUDY.

Popis výsledku anglicky

We have used molecular dynamics to help understanding different mechanism of CDK2 and CDK5 activation and inhibition. The data were obtained from several simulations including fully active CDK2 in a complex with a short peptide substrate. and simulationson CDK5 in complexes with the protein activator p25 and the purine-like inhibitor roscovitine. Differences between the CDK5/p25 and CDK2/Cyclin A systems are discussed with respect to their specific functionality. We will show why the CDK2 inhibition segment becomes an activation segment for CDK5. We will also clarify why only one step is necessary to activate CDK5 (interaction with the regulatory subunit) while CDK2 needs a two-step activation (other phosphorylation in the activation loop). As a ?sideeffect? information obtained from simulations, we will show how a detailed analysis of the interactions between the protein and the solvent could be used to design new inhibitors. This is especially important from the pharmacological poi

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GD204%2F03%2FH016" target="_blank" >GD204/03/H016: Strukturní biofyzika makromolekul</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

1st European Chemistry Congress

ISBN

9639319619

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

381-381

Název nakladatele

EuCheMS

Místo vydání

Budapest. Hungary

Místo konání akce

Budapest, Hungary

Datum konání akce

27. 8. 2006

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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