Studium strukturních změn laktoferinu pomocí prutokové injekční analýzy s elektrochemickou detekcí na povrchu elektrody ze skelného uhlíku

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00022107" target="_blank" >RIV/00216224:14310/07:00022107 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/07:00107893

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Study of structural changes of lactoferrin using flow injection analysis with electrochemical detection on the surface of glassy carbon electrode

Popis výsledku v původním jazyce

Lactoferrin is considered to be a multifunctional protein. It appears to play several biologically important roles, where its structure is very crucial. The aim of this work was to investigate the basic electrochemical behaviour of lactoferrin by both stationary and flow electrochemical methods with respect its structural changes under various denaturing conditions. The electroactivity of lactoferrin was studied by three various electrochemical methods (linear sweep, differential pulse and square wave voltammetry). Based on the results obtained, we utilized flow injection analysis with electrochemical detection (FIA ED) for determination of lactoferrin. We found out that the most suitable FIA ED conditions were as follows: working electrode potential of 900 mV, Britton Robinson buffer (pH 4.5) as the mobile phase, its flow rate 1 ml min 1. Finally, we attempted to follow the changes of lactoferrin signal in the presence of chemical compounds or under the physical conditions leading to

Název v anglickém jazyce

Study of structural changes of lactoferrin using flow injection analysis with electrochemical detection on the surface of glassy carbon electrode

Popis výsledku anglicky

Lactoferrin is considered to be a multifunctional protein. It appears to play several biologically important roles, where its structure is very crucial. The aim of this work was to investigate the basic electrochemical behaviour of lactoferrin by both stationary and flow electrochemical methods with respect its structural changes under various denaturing conditions. The electroactivity of lactoferrin was studied by three various electrochemical methods (linear sweep, differential pulse and square wave voltammetry). Based on the results obtained, we utilized flow injection analysis with electrochemical detection (FIA ED) for determination of lactoferrin. We found out that the most suitable FIA ED conditions were as follows: working electrode potential of 900 mV, Britton Robinson buffer (pH 4.5) as the mobile phase, its flow rate 1 ml min 1. Finally, we attempted to follow the changes of lactoferrin signal in the presence of chemical compounds or under the physical conditions leading to

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GC - Pěstování rostlin, osevní postupy

OECD FORD obor

—

Projekt

<a href="/cs/project/1M06030" target="_blank" >1M06030: Funkční genomika a proteomika ve šlechtění rostlin</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Chim. Slov.

ISSN

1318-0207

e-ISSN

—

Svazek periodika

54

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SI - Slovinská republika

Počet stran výsledku

6

Strana od-do

68-73

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—