Bacteria profiling by MALDI-MS: The influence of sample preparation on distinguishing closely related Pseudomonas species
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00022179" target="_blank" >RIV/00216224:14310/07:00022179 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Bacteria profiling by MALDI-MS: The influence of sample preparation on distinguishing closely related Pseudomonas species
Popis výsledku v původním jazyce
In this work we examined numerous influences of particular sample preparation steps to observe whether their variation may affect the final output of data processing.Direct MALDI-MS analysis of bacteria extracts with subsequent evaluation using spectra libraries represents a new and rapid technique for microorganism identification. Numerous laboratories have developed approaches leading to successful distinguishing of desired bacteria strains; however, there are several differences in described sample treatment protocols, which might delimit their universality.The genus Pseudomonas (Prokaryotes, Proteobacteria, Gammaproteobacteria) can be an example of environmentally and clinically important genus with complicated and evolving taxonomy and problematically distinguishable species. The genus is phylogenetically very heterogeneous and comprises species ubiquitous in soil and water ecosystems and important as plant, animal and human pathogens.
Název v anglickém jazyce
Bacteria profiling by MALDI-MS: The influence of sample preparation on distinguishing closely related Pseudomonas species
Popis výsledku anglicky
In this work we examined numerous influences of particular sample preparation steps to observe whether their variation may affect the final output of data processing.Direct MALDI-MS analysis of bacteria extracts with subsequent evaluation using spectra libraries represents a new and rapid technique for microorganism identification. Numerous laboratories have developed approaches leading to successful distinguishing of desired bacteria strains; however, there are several differences in described sample treatment protocols, which might delimit their universality.The genus Pseudomonas (Prokaryotes, Proteobacteria, Gammaproteobacteria) can be an example of environmentally and clinically important genus with complicated and evolving taxonomy and problematically distinguishable species. The genus is phylogenetically very heterogeneous and comprises species ubiquitous in soil and water ecosystems and important as plant, animal and human pathogens.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LC06034" target="_blank" >LC06034: Regulace morfogeneze rostlinných buněk a orgánů</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2007
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů