Chemotaxonomic Characterisation of Pseudomonads by MALDI-TOF MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00017393" target="_blank" >RIV/00216224:14310/06:00017393 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Chemotaxonomic Characterisation of Pseudomonads by MALDI-TOF MS

Popis výsledku v původním jazyce

Pseudomonads are wide-spread bacteria known for their degradation abilities. Bacteria of the genus are ubiquitous in soil, water and many other natural habitats. Their main importance lies in modification of enviroment (geochemical cycles, biodegradation). Some members of the genus are important pathogens of plants, animals and human. The genus Pseudomonas sensu stricto comprises 160 validly described species and 8 subspecies, at present. Classification of environmental Pseudomonas isolates is often complicated due to heterogeneity and genetic variability of the species. Analysis of intact bacteria cells by Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has been shown to provide characteristic peptide/protein profiles which enable rapid identification of the microorganisms.1 In our work, sample preparation procedures were optimised for this group of Gram-negative bacteria including culture preparation and conditions of MALDI-MS analysis.

Název v anglickém jazyce

Chemotaxonomic Characterisation of Pseudomonads by MALDI-TOF MS

Popis výsledku anglicky

Pseudomonads are wide-spread bacteria known for their degradation abilities. Bacteria of the genus are ubiquitous in soil, water and many other natural habitats. Their main importance lies in modification of enviroment (geochemical cycles, biodegradation). Some members of the genus are important pathogens of plants, animals and human. The genus Pseudomonas sensu stricto comprises 160 validly described species and 8 subspecies, at present. Classification of environmental Pseudomonas isolates is often complicated due to heterogeneity and genetic variability of the species. Analysis of intact bacteria cells by Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has been shown to provide characteristic peptide/protein profiles which enable rapid identification of the microorganisms.1 In our work, sample preparation procedures were optimised for this group of Gram-negative bacteria including culture preparation and conditions of MALDI-MS analysis.

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06034" target="_blank" >LC06034: Regulace morfogeneze rostlinných buněk a orgánů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů