Molekulární analýza genu frigifa (Fri) u pozdně kvetoucích rostlin Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00022293" target="_blank" >RIV/00216224:14310/07:00022293 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana

Popis výsledku v původním jazyce

Flowering time is stimulated by both environmental (light, temperature) and endogenous signals. FLC and FRI genes are the most important endogenous signals controlling onset of flowering in natural populations. FLC is a significant flowering repressor under the control of the FRI gene. Interaction of both genes is the reason of lateness in natural populations. In the FRI gene, two frequent deletions responsible for early-flowering phenotype, Ler- and Col-type are known. These deletions have been identified only in early-flowering plants but never in late-flowering plants. Therefore, our work was focused on molecular analysis of FRI gene in late-flowering ecotypes derived from natural populations in the Czech Republic (Je-4, Je-18, Je-27, Je-28 and Hod)and in four late-flowering mutants (dn, L4, Spi and M73). For this purpose five PCR primers that cover the complete sequence of the FRI gene including the promoter were used. Deletions or insertions were detected by agarose or polyacryla

Název v anglickém jazyce

Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana

Popis výsledku anglicky

Flowering time is stimulated by both environmental (light, temperature) and endogenous signals. FLC and FRI genes are the most important endogenous signals controlling onset of flowering in natural populations. FLC is a significant flowering repressor under the control of the FRI gene. Interaction of both genes is the reason of lateness in natural populations. In the FRI gene, two frequent deletions responsible for early-flowering phenotype, Ler- and Col-type are known. These deletions have been identified only in early-flowering plants but never in late-flowering plants. Therefore, our work was focused on molecular analysis of FRI gene in late-flowering ecotypes derived from natural populations in the Czech Republic (Je-4, Je-18, Je-27, Je-28 and Hod)and in four late-flowering mutants (dn, L4, Spi and M73). For this purpose five PCR primers that cover the complete sequence of the FRI gene including the promoter were used. Deletions or insertions were detected by agarose or polyacryla

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Plant biotechnology: impact on high quality plant reproduction

ISBN

978-80-89088-51-5

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

85

Název nakladatele

Institute of plant genetics and biotechnology, Slovak Republic

Místo vydání

Stará Lesná

Místo konání akce

Stará Lesná

Datum konání akce

1. 1. 2007

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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