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Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00022812" target="_blank" >RIV/00216224:14310/07:00022812 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/07:00087252

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical Sensing of Chromium-Induced DNA Damage: DNA Strand Breakage by Intermediates of Chromium(VI) Electrochemical Reduction.

Popis výsledku v původním jazyce

DNA damage by CrV and/or CrIV intermediates of CrVI electrochemical reduction was detected using a supercoiled DNA-modified mercury electrode. A signal sensitive to formation of DNA strand breaks, AC voltammetric DNA peak 3, increased due to incubation of the DNA-modified electrode in micromolar solutions of CrVI at potentials sufficiently negative for CrVI reduction. Damage to DNA in solutions containing CrVI and a chemical reductant (ascorbic acid, AA) was observed only at relatively high chromium concentrations (hundreds of M). To eliminate interferences of excess CrVI in measurements of guanine electrochemical signals, a magnetoseparation double surface electrochemical technique was introduced. Using this approach, DNA damage in solution was detected for 50-250 M CrVI upon addition of 1 mM AA. Our results suggest a more efficient DNA damage at the electrode surface due to continuous production of the reactive chromium species, compared to DNA exposure to chromium being reduced chem

Název v anglickém jazyce

Electrochemical Sensing of Chromium-Induced DNA Damage: DNA Strand Breakage by Intermediates of Chromium(VI) Electrochemical Reduction.

Popis výsledku anglicky

DNA damage by CrV and/or CrIV intermediates of CrVI electrochemical reduction was detected using a supercoiled DNA-modified mercury electrode. A signal sensitive to formation of DNA strand breaks, AC voltammetric DNA peak 3, increased due to incubation of the DNA-modified electrode in micromolar solutions of CrVI at potentials sufficiently negative for CrVI reduction. Damage to DNA in solutions containing CrVI and a chemical reductant (ascorbic acid, AA) was observed only at relatively high chromium concentrations (hundreds of M). To eliminate interferences of excess CrVI in measurements of guanine electrochemical signals, a magnetoseparation double surface electrochemical technique was introduced. Using this approach, DNA damage in solution was detected for 50-250 M CrVI upon addition of 1 mM AA. Our results suggest a more efficient DNA damage at the electrode surface due to continuous production of the reactive chromium species, compared to DNA exposure to chromium being reduced chem

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ELECTROANALYSIS

ISSN

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e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

x

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

2093-2102

Kód UT WoS článku

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EID výsledku v databázi Scopus

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