Serologické stanovení tularemie u myší použitím amperometrického imunosensoru

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00022879" target="_blank" >RIV/00216224:14310/07:00022879 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00000493:G41__/07:#0000250

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor

Popis výsledku v původním jazyce

Tularemia caused by the bacterium Francisella tularensis belongs to danger infective diseases, therefore timely diagnosis is an important part of protective activities. A novel approach for serological diagnostics of tularemia using a four-channel amperometric immunosensor was proposed and evaluated on BALB/c mice as a model organism. The immunosensor was able to work with small volumes of serum samples (0.05 L per assay) and diagnosed tularemic infection very early after immunization. From the 5th day,it was possible to distinguish tularemic sera from control sera from mice immunized with Escherichia coli on the probability level of 0.99 (t-test). Some 40 measurements per one hour can be realized with the developed procedure. The obtained results were confirmed by the standard indirect ELISA. The proposed immunosensor-based approach seems promising for rapid detection of microbial pathogen infections.

Název v anglickém jazyce

Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor

Popis výsledku anglicky

Tularemia caused by the bacterium Francisella tularensis belongs to danger infective diseases, therefore timely diagnosis is an important part of protective activities. A novel approach for serological diagnostics of tularemia using a four-channel amperometric immunosensor was proposed and evaluated on BALB/c mice as a model organism. The immunosensor was able to work with small volumes of serum samples (0.05 L per assay) and diagnosed tularemic infection very early after immunization. From the 5th day,it was possible to distinguish tularemic sera from control sera from mice immunized with Escherichia coli on the probability level of 0.99 (t-test). Some 40 measurements per one hour can be realized with the developed procedure. The obtained results were confirmed by the standard indirect ELISA. The proposed immunosensor-based approach seems promising for rapid detection of microbial pathogen infections.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/OSVTUO2006003" target="_blank" >OSVTUO2006003: BIODETEKCE - Studium nových metod detekce a identifikace bojových biologických prostředků</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

23

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

2507-2512

Kód UT WoS článku

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EID výsledku v databázi Scopus

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