Analysis of hormone-dependent de novo organogenesis in Arabidopsis thaliana.

Kód výsledku v IS VaVaI

RIV/00216224:14310/07:00041968 - isvavai.cz

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of hormone-dependent de novo organogenesis in Arabidopsis thaliana.

Popis výsledku v původním jazyce

Mutual interactions of cytokinins and auxins were identified as a major morphogenetic factor in plants. Molecular mechanisms are still largely unknown. Hypocotyls derived from ethiolated seedlings were affected by various concentration ratios of exogenous auxins and cytokinins. We analysed a role of both phytohormones in an ability of hypocotyl explants to form calli, SAM- and/or RAM-like structures. We applied two auxins, NAA and 2,4-D, differing in the mechanism of their intercellular transport, and auxin transport inhibitor NPA. WUS::NLS-GUS and CLV3::NLS-GUS marker lines were used to recognize the identity of newly formed organs and the role of both phytohormones in the regulation of the cytokinin-to-auxin ratio-dependent developmental switch. Changes in endogenous hormone maxima and position-specific hormone-dependent mitotic cell activity were studied. Suggested model of the cytokinin and auxin interactions during de novo organogenesis in Arabidopsis thaliana will be presented.

Název v anglickém jazyce

Analysis of hormone-dependent de novo organogenesis in Arabidopsis thaliana.

Popis výsledku anglicky

Mutual interactions of cytokinins and auxins were identified as a major morphogenetic factor in plants. Molecular mechanisms are still largely unknown. Hypocotyls derived from ethiolated seedlings were affected by various concentration ratios of exogenous auxins and cytokinins. We analysed a role of both phytohormones in an ability of hypocotyl explants to form calli, SAM- and/or RAM-like structures. We applied two auxins, NAA and 2,4-D, differing in the mechanism of their intercellular transport, and auxin transport inhibitor NPA. WUS::NLS-GUS and CLV3::NLS-GUS marker lines were used to recognize the identity of newly formed organs and the role of both phytohormones in the regulation of the cytokinin-to-auxin ratio-dependent developmental switch. Changes in endogenous hormone maxima and position-specific hormone-dependent mitotic cell activity were studied. Suggested model of the cytokinin and auxin interactions during de novo organogenesis in Arabidopsis thaliana will be presented.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

LC06034: Regulace morfogeneze rostlinných buněk a orgánů

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Z - Vyzkumny zamer (s odkazem do CEZ)
S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů