New insights into the early events of de novo organogenesis in Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F12%3A00057439" target="_blank" >RIV/00216224:14740/12:00057439 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

New insights into the early events of de novo organogenesis in Arabidopsis thaliana

Popis výsledku v původním jazyce

Plant cells are able to undergo postembryonic de novo organogenesis, and thus plant organs or an entire plant body are regenerated from previously differentiated cells. The long known phenomenon of in vitro plant regeneration suggests the principal roleof two phytohormones, cytokinin and auxin. The identity of newly formed organs was shown to be dependent on different concentration ratios of these two phytohormones. This work focuses on the influence of auxin/cytokinin interaction in the early stages of de novo organogenesis. We used different markers (members of the cytokinin signaling pathway, auxin accumulation and transport) in a hypocotyl experimental system. As experimental model we selected Arabidopsis mutant lines affected in cytokinin signaling: single and double mutants in cytokinin receptors AHK2, AHK3 and AHK4. Organogenic response of ahk mutant lines was compared with the wild type during the first five days of cultivation.

Název v anglickém jazyce

New insights into the early events of de novo organogenesis in Arabidopsis thaliana

Popis výsledku anglicky

Plant cells are able to undergo postembryonic de novo organogenesis, and thus plant organs or an entire plant body are regenerated from previously differentiated cells. The long known phenomenon of in vitro plant regeneration suggests the principal roleof two phytohormones, cytokinin and auxin. The identity of newly formed organs was shown to be dependent on different concentration ratios of these two phytohormones. This work focuses on the influence of auxin/cytokinin interaction in the early stages of de novo organogenesis. We used different markers (members of the cytokinin signaling pathway, auxin accumulation and transport) in a hypocotyl experimental system. As experimental model we selected Arabidopsis mutant lines affected in cytokinin signaling: single and double mutants in cytokinin receptors AHK2, AHK3 and AHK4. Organogenic response of ahk mutant lines was compared with the wild type during the first five days of cultivation.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP501%2F11%2F1150" target="_blank" >GAP501/11/1150: Význam interakcí cytokininů a auxinu v regulaci gravitropismu kořene u Arabidopsis thaliana.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů