Determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00050868" target="_blank" >RIV/00216224:14310/07:00050868 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/elan.200603738" target="_blank" >http://dx.doi.org/10.1002/elan.200603738</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.200603738" target="_blank" >10.1002/elan.200603738</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry

Popis výsledku v původním jazyce

The main aim of this work was to prepare and determine apoMT by adsorptive transfer stripping technique (AdTS) in connection with differential pulse voltammetry (DPV). Particularly, we investigated the electrochemical behavior of MT measured on the surface of hanging mercury drop electrode in the presence of sodium chloride as supporting electrolyte using AdTS DPV with respect to study the effects of MT signals by different concentrations and pH's of the electrolyte. Then, we aimed at utilizing this technique to observe changes in MTwhich are dependent on using strong chelating compounds ethylendiamine-N, N, N', N'-tetraacetic acid (EDTA). Thanks to ability of EDTA to bind heavy metals from active center of enzymes and regulation proteins, we were ableto prepare apoMT; that means, MT without any metal ion bound in its structure.

Název v anglickém jazyce

Determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry

Popis výsledku anglicky

The main aim of this work was to prepare and determine apoMT by adsorptive transfer stripping technique (AdTS) in connection with differential pulse voltammetry (DPV). Particularly, we investigated the electrochemical behavior of MT measured on the surface of hanging mercury drop electrode in the presence of sodium chloride as supporting electrolyte using AdTS DPV with respect to study the effects of MT signals by different concentrations and pH's of the electrolyte. Then, we aimed at utilizing this technique to observe changes in MTwhich are dependent on using strong chelating compounds ethylendiamine-N, N, N', N'-tetraacetic acid (EDTA). Thanks to ability of EDTA to bind heavy metals from active center of enzymes and regulation proteins, we were ableto prepare apoMT; that means, MT without any metal ion bound in its structure.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/LC06035" target="_blank" >LC06035: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

—

Svazek periodika

19

Číslo periodika v rámci svazku

2-3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

339-347

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—