Molecular analysis of repetitive elements in giant genomes of Fritillaria ( liliaceae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00025178" target="_blank" >RIV/00216224:14310/08:00025178 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular analysis of repetitive elements in giant genomes of Fritillaria ( liliaceae)

Popis výsledku v původním jazyce

The genus Fritillaria L. comprises taxa with the largest genomes so far reported for any plant species, and at the same time, it provides extensive infrageneric genome size variation (30,000-127,000 Mb/C) along with surprising karyotype uniformity. The genus has a bicontinental distribution (Eurasia, North America) and both diverged groups independently show a trend of increasing genome size with divergence. We have constructed fosmid genomic DNA libraries from one species of each group with approximately equal genome sizes (~45,000 Mb/C): Fritillaria imperialis (Eurasia) and F. affinis (North America). Both libraries were screened using total genomic DNA as a probe to identify clones containing highly repetitive elements. Four selected clones were sequenced and about 130 kb of repetitive sequences was characterized for each species.

Název v anglickém jazyce

Molecular analysis of repetitive elements in giant genomes of Fritillaria ( liliaceae)

Popis výsledku anglicky

The genus Fritillaria L. comprises taxa with the largest genomes so far reported for any plant species, and at the same time, it provides extensive infrageneric genome size variation (30,000-127,000 Mb/C) along with surprising karyotype uniformity. The genus has a bicontinental distribution (Eurasia, North America) and both diverged groups independently show a trend of increasing genome size with divergence. We have constructed fosmid genomic DNA libraries from one species of each group with approximately equal genome sizes (~45,000 Mb/C): Fritillaria imperialis (Eurasia) and F. affinis (North America). Both libraries were screened using total genomic DNA as a probe to identify clones containing highly repetitive elements. Four selected clones were sequenced and about 130 kb of repetitive sequences was characterized for each species.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA521%2F07%2F0284" target="_blank" >GA521/07/0284: Molekulární a cytogenetická analýza gigantických genomů rodu Fritillaria (Liliaceae)</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů