Structure-Function Relationships and Engineering of Haloalkane Dehalogenases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00026663" target="_blank" >RIV/00216224:14310/08:00026663 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Structure-Function Relationships and Engineering of Haloalkane Dehalogenases

Popis výsledku v původním jazyce

The structure?function relationships for haloalkane dehalogenases, representing one of the best characterized families of enzymes involved in degradation of halogenated compounds is described. A substantial amount of mechanistic and structural information is currently available on haloalkane dehalogenases, providing good theoretical framework for their modification by protein engineering. Examples of constructed mutants include variants with modified: (1) activity, (2) thermostability, (3) substrate specificity and (4) enantioselectivity. Some variants carried mutations in the tunnels, connecting the buried active site with surrounding solvent, rather in the active site itself. Mutagenesis in the residues lining the protein tunnels represents a new paradigm in protein engineering.

Název v anglickém jazyce

Structure-Function Relationships and Engineering of Haloalkane Dehalogenases

Popis výsledku anglicky

The structure?function relationships for haloalkane dehalogenases, representing one of the best characterized families of enzymes involved in degradation of halogenated compounds is described. A substantial amount of mechanistic and structural information is currently available on haloalkane dehalogenases, providing good theoretical framework for their modification by protein engineering. Examples of constructed mutants include variants with modified: (1) activity, (2) thermostability, (3) substrate specificity and (4) enantioselectivity. Some variants carried mutations in the tunnels, connecting the buried active site with surrounding solvent, rather in the active site itself. Mutagenesis in the residues lining the protein tunnels represents a new paradigm in protein engineering.

Druh

C - Kapitola v odborné knize

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06010" target="_blank" >LC06010: Centrum biokatalýzy a biotransformací</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Handbook of Hydrocarbon and Lipid Microbiology

ISBN

978-3-540-77584-3

Počet stran výsledku

18

Strana od-do

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Počet stran knihy

4480

Název nakladatele

Springer

Místo vydání

Heidelberg

Kód UT WoS kapitoly

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