Structure-Function Relationships and Engineering of Haloalkane Dehalogenases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00119131" target="_blank" >RIV/00216224:14310/19:00119131 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/978-3-319-50418-6_15" target="_blank" >https://doi.org/10.1007/978-3-319-50418-6_15</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-319-50418-6_15" target="_blank" >10.1007/978-3-319-50418-6_15</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structure-Function Relationships and Engineering of Haloalkane Dehalogenases

Popis výsledku v původním jazyce

The structure-function relationships for haloalkane dehalogenases, one of the best characterized enzyme families involved in degradation of halogenated compounds, are described. A substantial amount of mechanistic and structural information is currently available on haloalkane dehalogenases, providing good theoretical framework for their modification by protein engineering. Examples of constructed mutants include variants with modified (i) activity and specificity, (ii) stability, and (ii) enantioselectivity. Many variants carried mutations in the tunnels connecting the buried active site with surrounding solvent, rather than in the active site itself. Mutagenesis of residues lining the protein tunnels represents attractive and a viable approach of protein engineering.

Název v anglickém jazyce

Structure-Function Relationships and Engineering of Haloalkane Dehalogenases

Popis výsledku anglicky

The structure-function relationships for haloalkane dehalogenases, one of the best characterized enzyme families involved in degradation of halogenated compounds, are described. A substantial amount of mechanistic and structural information is currently available on haloalkane dehalogenases, providing good theoretical framework for their modification by protein engineering. Examples of constructed mutants include variants with modified (i) activity and specificity, (ii) stability, and (ii) enantioselectivity. Many variants carried mutations in the tunnels connecting the buried active site with surrounding solvent, rather than in the active site itself. Mutagenesis of residues lining the protein tunnels represents attractive and a viable approach of protein engineering.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Aerobic Utilization of Hydrocarbons, Oils, and Lipids

ISBN

9783319504179

Počet stran výsledku

21

Strana od-do

367-387

Počet stran knihy

859

Název nakladatele

Springer

Místo vydání

Cham

Kód UT WoS kapitoly

000623123900015