The effects of Eicosanoid biosynthesis inhibitors on oxidative stress, antioxidant response and protein profiles in hemolymph of Galleria mellonella larvae
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00042007" target="_blank" >RIV/00216224:14310/08:00042007 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The effects of Eicosanoid biosynthesis inhibitors on oxidative stress, antioxidant response and protein profiles in hemolymph of Galleria mellonella larvae
Popis výsledku v původním jazyce
Effects of eicosanoid biosynthesis inhibitors (EBIs) esculetin, dexamethasone and phenidone on hemolymph concentrations of the oxidative stress indicator malondialdehyde (MDA), antioxidant enzyme (glutathione S-transferase GST) and protein profiles of greater wax moth, Galleria mellonella (L.) seventh instar larvae were investigated. The insects were reared from first instars on artificial diets containing 0.001, 0.01, 0.1 and 1.0 g EBIs per 100 g of diets. MDA content was significantly increased in thepresence of esculetin. Low concentrations of esculetin led to increased GST activity. Dexamethasone at concentrations of 0.001 and 0.01% significantly resulted in increased hemolymph MDA content. However, high dietary dexamethasone concentrations causedsignificant decrease in MDA content while these concentrations resulted in increased GST activity by about three-fold. Dietary phenidone led to increased MDA content and GST activity in the larvae.
Název v anglickém jazyce
The effects of Eicosanoid biosynthesis inhibitors on oxidative stress, antioxidant response and protein profiles in hemolymph of Galleria mellonella larvae
Popis výsledku anglicky
Effects of eicosanoid biosynthesis inhibitors (EBIs) esculetin, dexamethasone and phenidone on hemolymph concentrations of the oxidative stress indicator malondialdehyde (MDA), antioxidant enzyme (glutathione S-transferase GST) and protein profiles of greater wax moth, Galleria mellonella (L.) seventh instar larvae were investigated. The insects were reared from first instars on artificial diets containing 0.001, 0.01, 0.1 and 1.0 g EBIs per 100 g of diets. MDA content was significantly increased in thepresence of esculetin. Low concentrations of esculetin led to increased GST activity. Dexamethasone at concentrations of 0.001 and 0.01% significantly resulted in increased hemolymph MDA content. However, high dietary dexamethasone concentrations causedsignificant decrease in MDA content while these concentrations resulted in increased GST activity by about three-fold. Dietary phenidone led to increased MDA content and GST activity in the larvae.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
ED - Fyziologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2008
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů