Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00043258" target="_blank" >RIV/00216224:14310/10:00043258 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L.

Popis výsledku v původním jazyce

We posed the hypothesis that eicosanoids also mediate antioxidant enzymatic defense reactions to pro-oxidant challenge. To test this idea, we reared first-instar larvae of Galleria mellonella (L.) with the lypoxygenase inhibitor, esculetin, the phospholipase A2 inhibitor,dexamethasone and the dual inhibitor of cyclooxygenase and lipoxygenase, phenidone to seventh-instars. Newly ecdysed seventh-instars were then fed on artificial diet containing xanthotoxin (XA) for 2 days. Treating seventh-instar larvaeof G. mellonella with XA induced lipid peroxidation and protein carbonylation as evident from the increased content of malondialdehyde (MDA) and protein carbonyls respectively, and antioxidative enzymatic response in a dose-dependent manner. Relative tocontrol, eicosanoid biosynthesis inhibitors also resulted in impaired MDA content and antioxidant enzyme activities. We infer from these findings that eicosanoids mediate insect antioxidant enzymatic responses to dietary pro-oxidants.

Název v anglickém jazyce

Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L.

Popis výsledku anglicky

We posed the hypothesis that eicosanoids also mediate antioxidant enzymatic defense reactions to pro-oxidant challenge. To test this idea, we reared first-instar larvae of Galleria mellonella (L.) with the lypoxygenase inhibitor, esculetin, the phospholipase A2 inhibitor,dexamethasone and the dual inhibitor of cyclooxygenase and lipoxygenase, phenidone to seventh-instars. Newly ecdysed seventh-instars were then fed on artificial diet containing xanthotoxin (XA) for 2 days. Treating seventh-instar larvaeof G. mellonella with XA induced lipid peroxidation and protein carbonylation as evident from the increased content of malondialdehyde (MDA) and protein carbonyls respectively, and antioxidative enzymatic response in a dose-dependent manner. Relative tocontrol, eicosanoid biosynthesis inhibitors also resulted in impaired MDA content and antioxidant enzyme activities. We infer from these findings that eicosanoids mediate insect antioxidant enzymatic responses to dietary pro-oxidants.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Comparative Biochemistry and Physiology A - Molecular & Integrative Physiology

ISSN

1095-6433

e-ISSN

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Svazek periodika

156

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000277748500003

EID výsledku v databázi Scopus

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