Modulation of gp130 Signalling by Inhibitors of Reactive Oxygen Species Formation in Mouse Embryonic Stem Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00030039" target="_blank" >RIV/00216224:14310/09:00030039 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Modulation of gp130 Signalling by Inhibitors of Reactive Oxygen Species Formation in Mouse Embryonic Stem Cells

Popis výsledku v původním jazyce

The gp130 system is a set of ligands and receptors that dominantly involves the activation of the JAK/STAT, Erk, and PI3K/Akt pathways. gp130 is suggested to play a key role in response of stem cells to both the mediators that maintain stem cell undifferentiated phenotype and the mediators inducing differentiation. Under physiological conditions in their niche, stem cells are kept in hypoxic microenvironment. Leaving their niche they are exposed to normal levels of oxygen. Thus reactive oxygen species (ROS) formation in stem cells due to hypoxic conditions could be considered as important regulator of stem cell fate. Here we analyzed effects of inhibitors of NADPH oxidases apocynin or diphenyleneiodonium (DPI), and a glutathione precursor N-acetyl-cysteine (NAC) on activity of gp130 signaling pathways and maintenance of mouse ES cells. The obtained results are compared with effects of ES cells culture in hypoxia condition.

Název v anglickém jazyce

Modulation of gp130 Signalling by Inhibitors of Reactive Oxygen Species Formation in Mouse Embryonic Stem Cells

Popis výsledku anglicky

The gp130 system is a set of ligands and receptors that dominantly involves the activation of the JAK/STAT, Erk, and PI3K/Akt pathways. gp130 is suggested to play a key role in response of stem cells to both the mediators that maintain stem cell undifferentiated phenotype and the mediators inducing differentiation. Under physiological conditions in their niche, stem cells are kept in hypoxic microenvironment. Leaving their niche they are exposed to normal levels of oxygen. Thus reactive oxygen species (ROS) formation in stem cells due to hypoxic conditions could be considered as important regulator of stem cell fate. Here we analyzed effects of inhibitors of NADPH oxidases apocynin or diphenyleneiodonium (DPI), and a glutathione precursor N-acetyl-cysteine (NAC) on activity of gp130 signaling pathways and maintenance of mouse ES cells. The obtained results are compared with effects of ES cells culture in hypoxia condition.

Druh

D - Stať ve sborníku

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

FREE RADICAL BIOLOGY AND MEDICINE

ISBN

—

ISSN

0891-5849

e-ISSN

—

Počet stran výsledku

1

Strana od-do

—

Název nakladatele

ELSEVIER SCIENCE INC

Místo vydání

New York, NY, USA

Místo konání akce

San Francisco, CA, USA

Datum konání akce

18. 11. 2009

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000271988500041