Cytokinins modulate auxin-induced organogenesis via regulation of the auxin efflux in Arabidopsis thaliana.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00043637" target="_blank" >RIV/00216224:14310/09:00043637 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Cytokinins modulate auxin-induced organogenesis via regulation of the auxin efflux in Arabidopsis thaliana.
Popis výsledku v původním jazyce
Auxin and cytokinins are principal regulators of the developmental fate of pluripotent plant cells. The molecular nature of their interaction during de novo organogenesis remains unclear. We used the hypocotyl explants-based in vitro system to study mechanisms underlying de novo organogenesis. We show that auxin, but not cytokinin, is able to induce organogenic response. Cytokinin modulates auxin-induced organogenesis (AIO) via regulation of intercellular auxin distribution. The AIO is accompanied withendogenous cytokinins production and tissue-specific activation of cytokinin signalling. Cytokinin regulates the expression of PIN auxin efflux carriers in hypocotyl explants. We analyzed PINs expression in roots of 6-day-old seedlings and found that PIN2 and PIN4 are downregulated in Pro35S:AtCKX2(3) lines. We propose a model in which auxin acts as a trigger of the organogenic processes, whose output is modulated by the endogenously produced CKs.
Název v anglickém jazyce
Cytokinins modulate auxin-induced organogenesis via regulation of the auxin efflux in Arabidopsis thaliana.
Popis výsledku anglicky
Auxin and cytokinins are principal regulators of the developmental fate of pluripotent plant cells. The molecular nature of their interaction during de novo organogenesis remains unclear. We used the hypocotyl explants-based in vitro system to study mechanisms underlying de novo organogenesis. We show that auxin, but not cytokinin, is able to induce organogenic response. Cytokinin modulates auxin-induced organogenesis (AIO) via regulation of intercellular auxin distribution. The AIO is accompanied withendogenous cytokinins production and tissue-specific activation of cytokinin signalling. Cytokinin regulates the expression of PIN auxin efflux carriers in hypocotyl explants. We analyzed PINs expression in roots of 6-day-old seedlings and found that PIN2 and PIN4 are downregulated in Pro35S:AtCKX2(3) lines. We propose a model in which auxin acts as a trigger of the organogenic processes, whose output is modulated by the endogenously produced CKs.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LC06034" target="_blank" >LC06034: Regulace morfogeneze rostlinných buněk a orgánů</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2009
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů