Cytokinins modulate auxin-induced organogenesis via regulation of the auxin transport

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00043640" target="_blank" >RIV/00216224:14310/09:00043640 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cytokinins modulate auxin-induced organogenesis via regulation of the auxin transport

Popis výsledku v původním jazyce

Auxin and cytokinin (CK) are principal regulators of the developmental fate of pluripotent plant cells. However, the molecular nature of their interaction during de novo organogenesis remains unclear. We used the hypocotyl explants-based in vitro systemto study mechanisms underlying de novo organogenesis. We show that auxin, but not CK, is able to induce organogenic response. CK modulates auxin-induced organogenesis (AIO) via regulation of intercellular auxin distribution. The AIO is accompanied with endogenous CKs production and tissue-specific activation of CK signalling. CK reduces auxin efflux from cultured tobacco cells and regulates the expression of PIN auxin efflux carriers in hypocotyl explants. We analyzed PINs expression in roots of 6-day-old seedlings and found that PIN2 and PIN4 are downregulated in Pro35S:AtCKX2(3) lines. We propose a model in which auxin acts as a trigger of the organogenic processes, whose output is modulated by the endogenously produced CKs.

Název v anglickém jazyce

Cytokinins modulate auxin-induced organogenesis via regulation of the auxin transport

Popis výsledku anglicky

Auxin and cytokinin (CK) are principal regulators of the developmental fate of pluripotent plant cells. However, the molecular nature of their interaction during de novo organogenesis remains unclear. We used the hypocotyl explants-based in vitro systemto study mechanisms underlying de novo organogenesis. We show that auxin, but not CK, is able to induce organogenic response. CK modulates auxin-induced organogenesis (AIO) via regulation of intercellular auxin distribution. The AIO is accompanied with endogenous CKs production and tissue-specific activation of CK signalling. CK reduces auxin efflux from cultured tobacco cells and regulates the expression of PIN auxin efflux carriers in hypocotyl explants. We analyzed PINs expression in roots of 6-day-old seedlings and found that PIN2 and PIN4 are downregulated in Pro35S:AtCKX2(3) lines. We propose a model in which auxin acts as a trigger of the organogenic processes, whose output is modulated by the endogenously produced CKs.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06034" target="_blank" >LC06034: Regulace morfogeneze rostlinných buněk a orgánů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů