COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00043372" target="_blank" >RIV/00216224:14310/10:00043372 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE
Popis výsledku v původním jazyce
Ascaridoid nematode species Brevimulticaecum heterotis obtained by parasitological dissection from the African bonytongue, Heterotis niloticus (Osteoglossidae) collected in Senegal has been chosen for studies by scanning electron microscope to redescribeits external morphology. Scanning electron microscopy (SEM) and environmental scanning electron microscopy (ESEM) were used for the study. For SEM, specimens were fixed with hot formalin. They were dehydrated, critical-point dried, mounted on stubs andcoated with gold. For ESEM, fixed specimens were hydrated with water; desiccation was not applied. Quanta 3D FEG and Quanta 250 FEG in SEM and ESEM mode were used. Combination of both techniques brings new level to nematode investigation. While SEM remains unsubstitutable method for study of nematode morphology due to its ease of use, ESEM is an unexceptionable instrument when there is only one unique specimen that cannot be destroyed or must remain available for molecular studies.
Název v anglickém jazyce
COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE
Popis výsledku anglicky
Ascaridoid nematode species Brevimulticaecum heterotis obtained by parasitological dissection from the African bonytongue, Heterotis niloticus (Osteoglossidae) collected in Senegal has been chosen for studies by scanning electron microscope to redescribeits external morphology. Scanning electron microscopy (SEM) and environmental scanning electron microscopy (ESEM) were used for the study. For SEM, specimens were fixed with hot formalin. They were dehydrated, critical-point dried, mounted on stubs andcoated with gold. For ESEM, fixed specimens were hydrated with water; desiccation was not applied. Quanta 3D FEG and Quanta 250 FEG in SEM and ESEM mode were used. Combination of both techniques brings new level to nematode investigation. While SEM remains unsubstitutable method for study of nematode morphology due to its ease of use, ESEM is an unexceptionable instrument when there is only one unique specimen that cannot be destroyed or must remain available for molecular studies.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EG - Zoologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů