Genomic diversity of two lineages of exfoliative toxin A-converting phages predominating in Staphylococcus aureus strains in Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00044089" target="_blank" >RIV/00216224:14310/10:00044089 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genomic diversity of two lineages of exfoliative toxin A-converting phages predominating in Staphylococcus aureus strains in Czech Republic

Popis výsledku v původním jazyce

We have isolated and characterized two distinct types of exfoliative toxin A (ETA) converting bacteriophages originated from the S. aureus strains responsible for massive outbreaks of pemphigus neonatorum in the Czech Republic. Three induced phages designated as phiB531, phiB557 and phiB122, were found to be capable of transferring the eta gene into the prophageless non-toxigenic S. aureus strain, and converting it into an ETA producer. Comparisons of the phage sequences derived from 12 selected genes and 2 genomic segments (polymorphic P2 and conserved C4) revealed that phiB531 and phiB557 were identical each other, but phiB122 differed from them in five gene sequences, the xis gene content, and virion protein profile. Thus, phiB122 represents a new type of still undescribed ETA converting phage. This study has highlighted not only the conclusive genomic diversity of eta gene-positive phages but also their virulence implications in impetigo S. aureus strains.

Název v anglickém jazyce

Genomic diversity of two lineages of exfoliative toxin A-converting phages predominating in Staphylococcus aureus strains in Czech Republic

Popis výsledku anglicky

We have isolated and characterized two distinct types of exfoliative toxin A (ETA) converting bacteriophages originated from the S. aureus strains responsible for massive outbreaks of pemphigus neonatorum in the Czech Republic. Three induced phages designated as phiB531, phiB557 and phiB122, were found to be capable of transferring the eta gene into the prophageless non-toxigenic S. aureus strain, and converting it into an ETA producer. Comparisons of the phage sequences derived from 12 selected genes and 2 genomic segments (polymorphic P2 and conserved C4) revealed that phiB531 and phiB557 were identical each other, but phiB122 differed from them in five gene sequences, the xis gene content, and virion protein profile. Thus, phiB122 represents a new type of still undescribed ETA converting phage. This study has highlighted not only the conclusive genomic diversity of eta gene-positive phages but also their virulence implications in impetigo S. aureus strains.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA310%2F09%2F0459" target="_blank" >GA310/09/0459: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>R - Projekt Ramcoveho programu EK<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Microbiology

ISSN

0923-2508

e-ISSN

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Svazek periodika

161

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000279125600003

EID výsledku v databázi Scopus

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