Transactivation by temperature dependent p53 mutants in yeast and human cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00044309" target="_blank" >RIV/00216224:14310/10:00044309 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Transactivation by temperature dependent p53 mutants in yeast and human cells

Popis výsledku v původním jazyce

In response to stress signals, p53 controls essential cell functions by regulating expression of its target genes. Full or partial loss of the p53 function in cancer cells usually results from mutations of the p53 gene. Some of them are temperature-dependent, allowing reactivation of the p53 function in certain temperature.We analyzed transactivation of p21-, bax- and mdm2 genes by 23 temperature-dependent p53 mutants in transiently transfected human lung H1299 cells (p53-null) by luciferase reporter assay.We confirmed temperature-dependency and discriminative character of the most p53 mutants and stratified them into four functional groups. Despite the differences of yeast and human cells, they allowed similar transactivation rates to the p53 mutants,thus providing evidence that functional analysis of separated alleles in yeast is valuable tool for assessment of the human p53 status.

Název v anglickém jazyce

Transactivation by temperature dependent p53 mutants in yeast and human cells

Popis výsledku anglicky

In response to stress signals, p53 controls essential cell functions by regulating expression of its target genes. Full or partial loss of the p53 function in cancer cells usually results from mutations of the p53 gene. Some of them are temperature-dependent, allowing reactivation of the p53 function in certain temperature.We analyzed transactivation of p21-, bax- and mdm2 genes by 23 temperature-dependent p53 mutants in transiently transfected human lung H1299 cells (p53-null) by luciferase reporter assay.We confirmed temperature-dependency and discriminative character of the most p53 mutants and stratified them into four functional groups. Despite the differences of yeast and human cells, they allowed similar transactivation rates to the p53 mutants,thus providing evidence that functional analysis of separated alleles in yeast is valuable tool for assessment of the human p53 status.

Druh

O - Ostatní výsledky

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů