Transactivation and reactivation capabilities of temperature-dependent p53 mutants in yeast and human cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00060467" target="_blank" >RIV/00216224:14310/12:00060467 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00209775:_____/12:#0000239 RIV/65269705:_____/12:#0001979

Výsledek na webu

<a href="http://dx.doi.org/10.3892/ijo.2012.1520" target="_blank" >http://dx.doi.org/10.3892/ijo.2012.1520</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/ijo.2012.1520" target="_blank" >10.3892/ijo.2012.1520</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Transactivation and reactivation capabilities of temperature-dependent p53 mutants in yeast and human cells

Popis výsledku v původním jazyce

The p53 protein is a sequence-specific transcription factor controlling the expression of multiple genes and protecting cells from oncogenic transformation. In many tumors, the p53 protein is completely or partially inactivated by mutations in the p53 gene. We analyzed the transactivating activity of nine human temperature-dependent (td) p53 mutants in yeast cells. Mutations in seven of them were localized in the beta-sandwich-coding region of the p53 gene, eight p53 mutants were temperature-sensitive and the R283C mutant was coldsensitive. Patterns of their transactivation abilities towards three different responsive elements, the extent of their temperature dependency as well as discriminativity, were considerably variable. Similarly, their capacityto become reactivated by amifostine varied from complete resistance to high sensitivity.

Název v anglickém jazyce

Transactivation and reactivation capabilities of temperature-dependent p53 mutants in yeast and human cells

Popis výsledku anglicky

The p53 protein is a sequence-specific transcription factor controlling the expression of multiple genes and protecting cells from oncogenic transformation. In many tumors, the p53 protein is completely or partially inactivated by mutations in the p53 gene. We analyzed the transactivating activity of nine human temperature-dependent (td) p53 mutants in yeast cells. Mutations in seven of them were localized in the beta-sandwich-coding region of the p53 gene, eight p53 mutants were temperature-sensitive and the R283C mutant was coldsensitive. Patterns of their transactivation abilities towards three different responsive elements, the extent of their temperature dependency as well as discriminativity, were considerably variable. Similarly, their capacityto become reactivated by amifostine varied from complete resistance to high sensitivity.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/NS10448" target="_blank" >NS10448: Podrobná analýza teplotně závislých mutantů nádorového supresoru p53 v lidských buňkách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Oncology

ISSN

1019-6439

e-ISSN

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Svazek periodika

41

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

7

Strana od-do

1157-1163

Kód UT WoS článku

000307150000042

EID výsledku v databázi Scopus

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