Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00045193" target="_blank" >RIV/00216224:14310/10:00045193 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS
Popis výsledku v původním jazyce
The human gastrointestinal tract contains a diverse microbial community. Among them lactobacilli are important members of the natural intestinal microbiota playing a beneficial role in balancing of the intestinal environment. A collection of 51 Lactobacillus isolates originating from children intestinal mucous tissues has been studied. Analysed biopsy samples were retrieved during a routine endoscopic examination of different parts of the large intestine in patients revealing various disorders. A combined approach, which included automated ribotyping and MALDI-TOF MS analysis, was applied for identification of isolated Lactobacillus spp. in order to compare the discriminatory power of these methods. Automated ribotyping using the RiboPrinter MicrobialCharacterization System (DuPont Qualicon) assigned 38 lactobacilli to the species level: Lactobacillus salivarius (29 isolates), Lactobacillus paracasei subsp. paracasei (7) and Lactobacillus rhamnosus (2).
Název v anglickém jazyce
Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS
Popis výsledku anglicky
The human gastrointestinal tract contains a diverse microbial community. Among them lactobacilli are important members of the natural intestinal microbiota playing a beneficial role in balancing of the intestinal environment. A collection of 51 Lactobacillus isolates originating from children intestinal mucous tissues has been studied. Analysed biopsy samples were retrieved during a routine endoscopic examination of different parts of the large intestine in patients revealing various disorders. A combined approach, which included automated ribotyping and MALDI-TOF MS analysis, was applied for identification of isolated Lactobacillus spp. in order to compare the discriminatory power of these methods. Automated ribotyping using the RiboPrinter MicrobialCharacterization System (DuPont Qualicon) assigned 38 lactobacilli to the species level: Lactobacillus salivarius (29 isolates), Lactobacillus paracasei subsp. paracasei (7) and Lactobacillus rhamnosus (2).
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů