Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00057485" target="_blank" >RIV/00216224:14310/10:00057485 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa

Popis výsledku v původním jazyce

Random mutagenesis is the most useful approach in directed evolution, widely used in protein engineering for producing proteins with altered or improved properties. Random mutagenesis includes a lot of methods used to create diverse mutant libraries. Oneof these methods is error-prone PCR. This technique introduces nucleotide changes during PCR due to the use of error-prone DNA polymerase and mutational reaction conditions. LecB is the target gene we have modified via random mutagenesis. This gene codes PA-IIL lectin from Pseudomonas aeruginosa. This lectin is well-characterized and it is indisputable that it displays an unusually high affinity for monosaccharides widely occurring as terminal residues in cell surface glycoconjugates highly present incystic fibrosis mucins. The random mutagenesis could help us to create a protein with interestingly modified properties towards monospecificity.

Název v anglickém jazyce

Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa

Popis výsledku anglicky

Random mutagenesis is the most useful approach in directed evolution, widely used in protein engineering for producing proteins with altered or improved properties. Random mutagenesis includes a lot of methods used to create diverse mutant libraries. Oneof these methods is error-prone PCR. This technique introduces nucleotide changes during PCR due to the use of error-prone DNA polymerase and mutational reaction conditions. LecB is the target gene we have modified via random mutagenesis. This gene codes PA-IIL lectin from Pseudomonas aeruginosa. This lectin is well-characterized and it is indisputable that it displays an unusually high affinity for monosaccharides widely occurring as terminal residues in cell surface glycoconjugates highly present incystic fibrosis mucins. The random mutagenesis could help us to create a protein with interestingly modified properties towards monospecificity.

Druh

O - Ostatní výsledky

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GD301%2F09%2FH004" target="_blank" >GD301/09/H004: Molekulární a strukturní biologie vybraných cytostatik. Od mechanistických studií k chemoterapii rakoviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů