Protein engineering of lectins - in silico and in vitro approaches

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F11%3A00050180" target="_blank" >RIV/00216224:14740/11:00050180 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Protein engineering of lectins - in silico and in vitro approaches

Popis výsledku v původním jazyce

rotein-carbohydrate interactions are usually characterized by a low affinity for monovalent ligands that is balanced by multivalency resulting in high avidity for ligands with several potential epitops. However, recent characterization of lectins involved in pathogenesis has demonstrated their much higher affinity even towards monosaccharide than that observed for plant or animal lectins [1]. This feature allows to use such lectins as a more/less specific tool, for example, for evaluation of protein glycosylation or isolation of glyco-conjugates. Random mutagenesis is often used in protein engineering for producing proteins with altered or improved properties. In our work several methodical directions have been applied. Error-prone PCR is based on error-prone DNA polymerase which introduces nucleotide changes during extension of amplified DNA.

Název v anglickém jazyce

Protein engineering of lectins - in silico and in vitro approaches

Popis výsledku anglicky

rotein-carbohydrate interactions are usually characterized by a low affinity for monovalent ligands that is balanced by multivalency resulting in high avidity for ligands with several potential epitops. However, recent characterization of lectins involved in pathogenesis has demonstrated their much higher affinity even towards monosaccharide than that observed for plant or animal lectins [1]. This feature allows to use such lectins as a more/less specific tool, for example, for evaluation of protein glycosylation or isolation of glyco-conjugates. Random mutagenesis is often used in protein engineering for producing proteins with altered or improved properties. In our work several methodical directions have been applied. Error-prone PCR is based on error-prone DNA polymerase which introduces nucleotide changes during extension of amplified DNA.

Druh

O - Ostatní výsledky

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů