Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00049447" target="_blank" >RIV/00216224:14310/11:00049447 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67179843:_____/11:00372340 RIV/60076658:12520/11:43882929

Výsledek na webu

<a href="http://dx.doi.org/10.1107/S1744309111001254" target="_blank" >http://dx.doi.org/10.1107/S1744309111001254</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1107/S1744309111001254" target="_blank" >10.1107/S1744309111001254</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane

Popis výsledku v původním jazyce

Haloalkane dehalogenases hydrolyze carbon-halogen bonds in a wide range of halogenated aliphatic compounds. The potential use of haloalkane dehalogenases in bioremediation applications has stimulated intensive investigation of these enzymes and their engineering. The mutant DhaA31 was constructed to degrade the anthropogenic compound 1,2,3-trichloropropane (TCP) using a new strategy. This strategy enhances activity towards TCP by decreasing the accessibility of the active site to water molecules, thereby promoting formation of the activated complex. The structure of DhaA31 will help in understanding the structure-function relationships involved in the improved dehalogenation of TCP. The mutant protein DhaA31 was crystallized by the sitting-drop vapour-diffusion technique and crystals of DhaA31 in complex with TCP were obtained using soaking experiments. Both crystals belonged to the triclinic space group P1.

Název v anglickém jazyce

Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane

Popis výsledku anglicky

Haloalkane dehalogenases hydrolyze carbon-halogen bonds in a wide range of halogenated aliphatic compounds. The potential use of haloalkane dehalogenases in bioremediation applications has stimulated intensive investigation of these enzymes and their engineering. The mutant DhaA31 was constructed to degrade the anthropogenic compound 1,2,3-trichloropropane (TCP) using a new strategy. This strategy enhances activity towards TCP by decreasing the accessibility of the active site to water molecules, thereby promoting formation of the activated complex. The structure of DhaA31 will help in understanding the structure-function relationships involved in the improved dehalogenation of TCP. The mutant protein DhaA31 was crystallized by the sitting-drop vapour-diffusion technique and crystals of DhaA31 in complex with TCP were obtained using soaking experiments. Both crystals belonged to the triclinic space group P1.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Crystallographica F

ISSN

1744-3091

e-ISSN

—

Svazek periodika

67

Číslo periodika v rámci svazku

67

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

397-400

Kód UT WoS článku

000288225100022

EID výsledku v databázi Scopus

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