OVEREXPRESSION OF CATHEPSIN D SENZITIZES BREAST CANCER CELLS TO TRAIL-INDUCED APOPTOSIS
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00049487" target="_blank" >RIV/00216224:14310/11:00049487 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
OVEREXPRESSION OF CATHEPSIN D SENZITIZES BREAST CANCER CELLS TO TRAIL-INDUCED APOPTOSIS
Popis výsledku v původním jazyce
Breast cancer cells overexpressing cathepsin D wt and mut were exposed to TRAIL and frequency of apoptosis was determined by chromatin condensation, nuclear fragmentation, cleavage of PARP, and externalization of phosphatidylserine. An increased frequency of apoptosis in cells over-expressing wt but not mut CTSD was clearly demonstrated. Increased frequency of apoptosis observed in wt CTSD cells corresponded to cleavage of the pro-apoptotic Bcl-2 family member, the Bid protein, that was earlier identified as one of the targets of CTSD in cancer cells. We conclude that CTSD sensitizes MDA-MB-231 cells to the TRAIL-induced apoptosis in enzymatic activity-dependent manner and that the TRAIL-induced apoptosis is potentiated by the CTSD-mediated activationof Bid, the pro-apoptotic member of the Bcl-2 family.
Název v anglickém jazyce
OVEREXPRESSION OF CATHEPSIN D SENZITIZES BREAST CANCER CELLS TO TRAIL-INDUCED APOPTOSIS
Popis výsledku anglicky
Breast cancer cells overexpressing cathepsin D wt and mut were exposed to TRAIL and frequency of apoptosis was determined by chromatin condensation, nuclear fragmentation, cleavage of PARP, and externalization of phosphatidylserine. An increased frequency of apoptosis in cells over-expressing wt but not mut CTSD was clearly demonstrated. Increased frequency of apoptosis observed in wt CTSD cells corresponded to cleavage of the pro-apoptotic Bcl-2 family member, the Bid protein, that was earlier identified as one of the targets of CTSD in cancer cells. We conclude that CTSD sensitizes MDA-MB-231 cells to the TRAIL-induced apoptosis in enzymatic activity-dependent manner and that the TRAIL-induced apoptosis is potentiated by the CTSD-mediated activationof Bid, the pro-apoptotic member of the Bcl-2 family.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů