A Study of Cytosine Oligonucleotides by Voltammetric and Spectral Methods
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00053671" target="_blank" >RIV/00216224:14310/11:00053671 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
A Study of Cytosine Oligonucleotides by Voltammetric and Spectral Methods
Popis výsledku v původním jazyce
For the study of three short homo?deoxyoligonucleotides (homo?ODNs) containing cytosine ? dC3 , dC6 and dC9 ? we used electrochemical and spectral methods. Using linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS) the effect of pH and the number of C in the ODN chain on reduction signals in buffered solutions was investigated.1-2 The reduction C peaks, recorded at hanging mercury drop electrode (HMDE) in the potential range from ?1.0 V to ?1.7 V vs. Ag/AgCl/3 M KCl, are strongly dependent not only on pH but also on the composition of solutions. The measurements were done in phosphate ? acetate buffer (0.08 M) with the addition of 0.1M NaCl in pH range from 1.55 to 8.57. The concentration of all ODN was 10 uM. From acidicto alkaline pH scale, three reduction ranges were found. For dC3, dC6 and dC9 we observe on voltammetric curves a shift of the reduction peaks towards more negative potentials with increasing pH and increasing scan rate.
Název v anglickém jazyce
A Study of Cytosine Oligonucleotides by Voltammetric and Spectral Methods
Popis výsledku anglicky
For the study of three short homo?deoxyoligonucleotides (homo?ODNs) containing cytosine ? dC3 , dC6 and dC9 ? we used electrochemical and spectral methods. Using linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS) the effect of pH and the number of C in the ODN chain on reduction signals in buffered solutions was investigated.1-2 The reduction C peaks, recorded at hanging mercury drop electrode (HMDE) in the potential range from ?1.0 V to ?1.7 V vs. Ag/AgCl/3 M KCl, are strongly dependent not only on pH but also on the composition of solutions. The measurements were done in phosphate ? acetate buffer (0.08 M) with the addition of 0.1M NaCl in pH range from 1.55 to 8.57. The concentration of all ODN was 10 uM. From acidicto alkaline pH scale, three reduction ranges were found. For dC3, dC6 and dC9 we observe on voltammetric curves a shift of the reduction peaks towards more negative potentials with increasing pH and increasing scan rate.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
CF - Fyzikální chemie a teoretická chemie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LC06035" target="_blank" >LC06035: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů