Electrochemical Biosensor for Detection of Bioagents

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00055984" target="_blank" >RIV/00216224:14310/11:00055984 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00000493:G41__/11:#0000386

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol6/6125968.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol6/6125968.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical Biosensor for Detection of Bioagents

Popis výsledku v původním jazyce

Simple and rapid detection and identification of dangerous bioagents is important for preventing illness or even death of people due to infectious diseases and bioterrorist threats. Electrochemical detection has a large potential for development of portable instrumentation suitable for analysis in field and especially combination with specificity of immunoassays seems promising. Initially, the construction of the immunosensing layer was realised and the effect of Au nanoparticles on the response of peroxidase was studied. An amperometric immunosensor was developed using screen-printed transducer, it employed specific capture of microbes in the sensing area by formation of an immunocomplex and its subsequent labelling using the tracer - antibody conjugated to peroxidase. The obtained sandwich electrochemical immunoassay was applied for the model microbial cells of B. subtilis. The total time of analysis was 20 min and the detection of cells below 104 CFU/ml was feasible.

Název v anglickém jazyce

Electrochemical Biosensor for Detection of Bioagents

Popis výsledku anglicky

Simple and rapid detection and identification of dangerous bioagents is important for preventing illness or even death of people due to infectious diseases and bioterrorist threats. Electrochemical detection has a large potential for development of portable instrumentation suitable for analysis in field and especially combination with specificity of immunoassays seems promising. Initially, the construction of the immunosensing layer was realised and the effect of Au nanoparticles on the response of peroxidase was studied. An amperometric immunosensor was developed using screen-printed transducer, it employed specific capture of microbes in the sensing area by formation of an immunocomplex and its subsequent labelling using the tracer - antibody conjugated to peroxidase. The obtained sandwich electrochemical immunoassay was applied for the model microbial cells of B. subtilis. The total time of analysis was 20 min and the detection of cells below 104 CFU/ml was feasible.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

12

Strana od-do

5968-5979

Kód UT WoS článku

000297571400002

EID výsledku v databázi Scopus

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