First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F23%3A00576143" target="_blank" >RIV/61388955:_____/23:00576143 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10472904

Výsledek na webu

<a href="https://hdl.handle.net/11104/0345735" target="_blank" >https://hdl.handle.net/11104/0345735</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2023.109403" target="_blank" >10.1016/j.microc.2023.109403</a>

Jazyk výsledku

angličtina

Název v původním jazyce

First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6

Popis výsledku v původním jazyce

A novel electrochemical bioplatform for Pru du 6 determination, one of the most abundant and allergenic almond proteins, was developed to detect traces of almonds in processed foods. The bioplatform combines the application of a sandwich immunoassay on the surface of magnetic beads using specific antibodies unmodified or conjugated to horseradish peroxidase as capture and detection antibodies. The resulting magnetic bioconjugates are trapped on the surface of disposable carbon electrodes, and amperometry in the presence of hydrogen peroxide and hydroquinone is employed to measure the change in the cathodic current, which is proportional to Pru du 6 concentration. The developed bioplatform exhibited good selectivity and sensitivity, offering a detection limit of 0.12 ng mL−1 of Pru du 6. It could determine Pru du 6 in raw dough and baked cookies incurred with 0.2 µg g−1 of almond protein with acceptable precision, providing results comparable to those obtained using an ELISA technique.

Název v anglickém jazyce

First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6

Popis výsledku anglicky

A novel electrochemical bioplatform for Pru du 6 determination, one of the most abundant and allergenic almond proteins, was developed to detect traces of almonds in processed foods. The bioplatform combines the application of a sandwich immunoassay on the surface of magnetic beads using specific antibodies unmodified or conjugated to horseradish peroxidase as capture and detection antibodies. The resulting magnetic bioconjugates are trapped on the surface of disposable carbon electrodes, and amperometry in the presence of hydrogen peroxide and hydroquinone is employed to measure the change in the cathodic current, which is proportional to Pru du 6 concentration. The developed bioplatform exhibited good selectivity and sensitivity, offering a detection limit of 0.12 ng mL−1 of Pru du 6. It could determine Pru du 6 in raw dough and baked cookies incurred with 0.2 µg g−1 of almond protein with acceptable precision, providing results comparable to those obtained using an ELISA technique.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

195

Číslo periodika v rámci svazku

DEC 2023

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

109403

Kód UT WoS článku

001097443400001

EID výsledku v databázi Scopus

2-s2.0-85172704832