Utilization of the anodic electro-osmotic flow for an amino acids profiling by capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00057816" target="_blank" >RIV/00216224:14310/12:00057816 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ce-ce.org/cece2012/Proceedings%20CECE%202012%20Final+late%20abstracts_web.pdf" target="_blank" >http://www.ce-ce.org/cece2012/Proceedings%20CECE%202012%20Final+late%20abstracts_web.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Utilization of the anodic electro-osmotic flow for an amino acids profiling by capillary electrophoresis

Popis výsledku v původním jazyce

Amino acid profiling is sometimes considered to be a supplementary analysis for diagnostic purposes. Detection of amino acids by conventional spectrophotometric techniques is hindered by lack of chromophores in their structures, thus derivatization stepin a sample treatment protocol has to occur. However, amino acids in their native form can be detected by other detection techniques, e.g. mass spectrometry or capacitively coupled contactless conductivity detection (C4D). C4D is a non specific detectiontechnique suitable for detection of ionic species and was successfully used for determination of amino acids. Completely resolved peaks were obtained only when long capillaries and electro-osmotic flow (EOF) suppression were applied. However, effectiveseparation time of analytes of interest can be altered by EOF modification. Induction of the anodic EOF for amino acid profiling in the shorter capillaries is the subject of presented contribution.

Název v anglickém jazyce

Utilization of the anodic electro-osmotic flow for an amino acids profiling by capillary electrophoresis

Popis výsledku anglicky

Amino acid profiling is sometimes considered to be a supplementary analysis for diagnostic purposes. Detection of amino acids by conventional spectrophotometric techniques is hindered by lack of chromophores in their structures, thus derivatization stepin a sample treatment protocol has to occur. However, amino acids in their native form can be detected by other detection techniques, e.g. mass spectrometry or capacitively coupled contactless conductivity detection (C4D). C4D is a non specific detectiontechnique suitable for detection of ionic species and was successfully used for determination of amino acids. Completely resolved peaks were obtained only when long capillaries and electro-osmotic flow (EOF) suppression were applied. However, effectiveseparation time of analytes of interest can be altered by EOF modification. Induction of the anodic EOF for amino acid profiling in the shorter capillaries is the subject of presented contribution.

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

9th International Interdisciplinary Meeting on Bioanalysis

ISBN

9788090495913

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

235-239

Název nakladatele

Institute of Analytical Chemistry AS CR, v. v. i., Veveří 97, 602 00 Brno

Místo vydání

Brno, Czech Republic

Místo konání akce

Hotel Continental, Brno, Czech Republic

Datum konání akce

1. 11. 2012

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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