Altered glutathione pool in response to short-term high light stress: Is there any interspecific difference in extremophilic lichens?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00060776" target="_blank" >RIV/00216224:14310/12:00060776 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Altered glutathione pool in response to short-term high light stress: Is there any interspecific difference in extremophilic lichens?

Popis výsledku v původním jazyce

Glutathione is a natural tripeptide with several functions in the plants. Among them, the ability to protect plant cells against various abiotic and biotic stresses is of most importance. In our laboratory, glutathione and its response to high light stress has been studied for a several years. It is generally known that short-term exposure to high light stress decreases glutathione pool (GSH) and increases oxidized form (GSSG). In this study, we focused on glutathione redox state in extremophilic lichenspecies (e.g. Usnea antartica, Usnea -2 -1 aurantiaco-atra, Pseudocyphellaria endochrysa) exposed to 800 mol.m .s (HL, high light). Glutathione was extracted in HCl and determined by labeling by thiol-binding fluorescence pigment monobromobimane in samples taken before light treatment and after the -2 -1 exposure lasting 10, 20, 30, 40, 50, 60 min., and during dark (11 mol.m .s ) recovery. HPLC evaluation of glutathione redox state was made on HPLC (Waters, USA).

Název v anglickém jazyce

Altered glutathione pool in response to short-term high light stress: Is there any interspecific difference in extremophilic lichens?

Popis výsledku anglicky

Glutathione is a natural tripeptide with several functions in the plants. Among them, the ability to protect plant cells against various abiotic and biotic stresses is of most importance. In our laboratory, glutathione and its response to high light stress has been studied for a several years. It is generally known that short-term exposure to high light stress decreases glutathione pool (GSH) and increases oxidized form (GSSG). In this study, we focused on glutathione redox state in extremophilic lichenspecies (e.g. Usnea antartica, Usnea -2 -1 aurantiaco-atra, Pseudocyphellaria endochrysa) exposed to 800 mol.m .s (HL, high light). Glutathione was extracted in HCl and determined by labeling by thiol-binding fluorescence pigment monobromobimane in samples taken before light treatment and after the -2 -1 exposure lasting 10, 20, 30, 40, 50, 60 min., and during dark (11 mol.m .s ) recovery. HPLC evaluation of glutathione redox state was made on HPLC (Waters, USA).

Druh

O - Ostatní výsledky

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/LM2010009" target="_blank" >LM2010009: Projekt CzechPolar - České polární stanice: Stavba a operační náklady</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů