Drosophila immune responses against entomopathogenic nematodes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00060848" target="_blank" >RIV/00216224:14310/12:00060848 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Drosophila immune responses against entomopathogenic nematodes

Popis výsledku v původním jazyce

Entomopathogenic nematodes (EPN?s) of the genera Heterorhabditis are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. Infective juveniles occur free living in the soil and are capable ofseeking out hosts and penetrate them through the cuticle or natural orifices. EPN?s are symbiotically associated with bacteria of the genera Photorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest host tissues. Drosophila larvae are more resistant to nematode infection than Galleria mellonella, but both can be used as natural infection model. The tripartite model (Drosophila, nematodes, bacteria) was recently established and used to show an immune function for transglutaminase, a conserved clotting factor. In this study we optimized the use of Drosophila as hosts and used different Drosophila mutants or RNAi lines with defects in clotting or other branches of the immune system.

Název v anglickém jazyce

Drosophila immune responses against entomopathogenic nematodes

Popis výsledku anglicky

Entomopathogenic nematodes (EPN?s) of the genera Heterorhabditis are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. Infective juveniles occur free living in the soil and are capable ofseeking out hosts and penetrate them through the cuticle or natural orifices. EPN?s are symbiotically associated with bacteria of the genera Photorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest host tissues. Drosophila larvae are more resistant to nematode infection than Galleria mellonella, but both can be used as natural infection model. The tripartite model (Drosophila, nematodes, bacteria) was recently established and used to show an immune function for transglutaminase, a conserved clotting factor. In this study we optimized the use of Drosophila as hosts and used different Drosophila mutants or RNAi lines with defects in clotting or other branches of the immune system.

Druh

O - Ostatní výsledky

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů