Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00062930" target="_blank" >RIV/00216224:14310/12:00062930 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.snb.2011.09.046" target="_blank" >http://dx.doi.org/10.1016/j.snb.2011.09.046</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2011.09.046" target="_blank" >10.1016/j.snb.2011.09.046</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing

Popis výsledku v původním jazyce

An approach for the immobilization of a pH indicator in optical pH sensors and biosensors was developed. Fluorescent dyes 5(6)-carboxyfluorescein and 5(6)-carboxynaphthofluorescein were conjugated with bovine serum albumin as a non-enzymatic scaffold protein and the conjugation procedure was optimized. Fluorescent properties, sensitivity to temperature and photostability of conjugates have been studied and characterized into details. The conjugates were immobilized on glass support by: (i) glutaraldehyde crosslinking or (ii) entrapment in sol?gel matrix ORMOCER with subsequent glutaraldehyde cross-linking. The response to pH and leaching potential were evaluated. The sensor layer, based on immobilized conjugates of 5(6)-carboxyfluorescein and bovine serum albumin, displayed rapid response over the pH range 4.0?9.0, making it compatible with a range of applications such as bioprocessing, clinical diagnostics or environmental monitoring.

Název v anglickém jazyce

Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing

Popis výsledku anglicky

An approach for the immobilization of a pH indicator in optical pH sensors and biosensors was developed. Fluorescent dyes 5(6)-carboxyfluorescein and 5(6)-carboxynaphthofluorescein were conjugated with bovine serum albumin as a non-enzymatic scaffold protein and the conjugation procedure was optimized. Fluorescent properties, sensitivity to temperature and photostability of conjugates have been studied and characterized into details. The conjugates were immobilized on glass support by: (i) glutaraldehyde crosslinking or (ii) entrapment in sol?gel matrix ORMOCER with subsequent glutaraldehyde cross-linking. The response to pH and leaching potential were evaluated. The sensor layer, based on immobilized conjugates of 5(6)-carboxyfluorescein and bovine serum albumin, displayed rapid response over the pH range 4.0?9.0, making it compatible with a range of applications such as bioprocessing, clinical diagnostics or environmental monitoring.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED0001%2F01%2F01" target="_blank" >ED0001/01/01: CETOCOEN</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

SENSORS AND ACTUATORS B: CHEMICAL

ISSN

0925-4005

e-ISSN

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Svazek periodika

161

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

7

Strana od-do

93-99

Kód UT WoS článku

000301549400007

EID výsledku v databázi Scopus

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