Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00065568" target="_blank" >RIV/00216224:14310/13:00065568 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1758-2229.2012.00378.x/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/j.1758-2229.2012.00378.x/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1758-2229.2012.00378.x" target="_blank" >10.1111/j.1758-2229.2012.00378.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies

Popis výsledku v původním jazyce

Staphylococcus aureus is a serious human and veterinary pathogen in which new strains with increasing virulence and antimicrobial resistance occur due to acquiring new genes by horizontal transfer. It is generally accepted that temperate bacteriophages play a major role in gene transfer. In this study, we proved the presence of various bacterial genes of the S. aureus COL strain directly within the phage particles via qPCR and quantified their packaging frequency. Nonparametric statistical analysis showed that transducing bacteriophages 11, 80 and 80lpha of serogroup B, in contrast to serogroup A bacteriophage 81, efficiently package selected chromosomal genes localized in 4 various loci of the chromosome and 8 genes carried on variable elements like staphylococcal cassette chromosome SCCmec, staphylococcal pathogenicity island SaPI1, genomic islands Saalpha and Sabeta, and plasmids with various frequency. Bacterial gene copy number per ng of DNA isolated from phage particles ranged be

Název v anglickém jazyce

Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies

Popis výsledku anglicky

Staphylococcus aureus is a serious human and veterinary pathogen in which new strains with increasing virulence and antimicrobial resistance occur due to acquiring new genes by horizontal transfer. It is generally accepted that temperate bacteriophages play a major role in gene transfer. In this study, we proved the presence of various bacterial genes of the S. aureus COL strain directly within the phage particles via qPCR and quantified their packaging frequency. Nonparametric statistical analysis showed that transducing bacteriophages 11, 80 and 80lpha of serogroup B, in contrast to serogroup A bacteriophage 81, efficiently package selected chromosomal genes localized in 4 various loci of the chromosome and 8 genes carried on variable elements like staphylococcal cassette chromosome SCCmec, staphylococcal pathogenicity island SaPI1, genomic islands Saalpha and Sabeta, and plasmids with various frequency. Bacterial gene copy number per ng of DNA isolated from phage particles ranged be

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Microbiology Reports

ISSN

1758-2229

e-ISSN

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Svazek periodika

5

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

66-73

Kód UT WoS článku

000314474500006

EID výsledku v databázi Scopus

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