Application of capillary electrophoresis for high-throughput screening of drug metabolism

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00066018" target="_blank" >RIV/00216224:14310/13:00066018 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/9781118530009.ch17" target="_blank" >http://dx.doi.org/10.1002/9781118530009.ch17</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/9781118530009.ch17" target="_blank" >10.1002/9781118530009.ch17</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of capillary electrophoresis for high-throughput screening of drug metabolism

Popis výsledku v původním jazyce

Pharmaceutical industry currently needs to screen extensive compound libraries within pre-clinical stages of drug development. Capillary electrophoresis has become a well-established analytical technique during the last two decades. In spite of the factthat capillary electrophoresis offers a number of favorable features for high-throughput analytical assays, it is still less important than chromatographic techniques in drug development programs. Hence, the main aim of this chapter is to discuss the practical limitations of capillary electrophoresis utilization and suggest ways to overcome these constraints. This is illustrated with a practical example namely the introduction of a method allowing rapid characterization of a compound?s stability againsthuman liver microsomes. NADP+ production monitoring was adopted to ensure generic applicability of the method.

Název v anglickém jazyce

Application of capillary electrophoresis for high-throughput screening of drug metabolism

Popis výsledku anglicky

Pharmaceutical industry currently needs to screen extensive compound libraries within pre-clinical stages of drug development. Capillary electrophoresis has become a well-established analytical technique during the last two decades. In spite of the factthat capillary electrophoresis offers a number of favorable features for high-throughput analytical assays, it is still less important than chromatographic techniques in drug development programs. Hence, the main aim of this chapter is to discuss the practical limitations of capillary electrophoresis utilization and suggest ways to overcome these constraints. This is illustrated with a practical example namely the introduction of a method allowing rapid characterization of a compound?s stability againsthuman liver microsomes. NADP+ production monitoring was adopted to ensure generic applicability of the method.

Druh

C - Kapitola v odborné knize

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Capillary Electrophoresis and Microchip Capillary Electrophoresis: Principles, Applications, and Limitations

ISBN

9780470572177

Počet stran výsledku

9

Strana od-do

309-317

Počet stran knihy

416

Název nakladatele

John Wiley & Sons, Inc.

Místo vydání

Hoboken, New Jersey

Kód UT WoS kapitoly

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