Comparison of cystatins of four species of helmints

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00066891" target="_blank" >RIV/00216224:14310/13:00066891 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of cystatins of four species of helmints

Popis výsledku v původním jazyce

Cystatins, the inhibitors of cysteine peptidases, are expressed and released by many parasitic organisms. They are participating on regulation of biological processes, e.g., the modification of the host immune response. Our study is primarily focused oncharacterization of molecular, biochemical and immunological properties of cystatins of four model species representing the four main groups of helmints: Fascioloides magna (Trematoda), Hymenolepis diminuta (Cestoda), Diplozoon paradoxum (Monogenea) andTrichinella spiralis (Nematoda). The excretory-secretory products were isolated, purified and fractionated and the identification of cystatins is in process. The RNA was isolated, reverse transcription performed and prepared cDNA used in PCR with specific primers designed for amplification of particular cystatine genes. Obtained amplicons will be sequenced and the selected cystatine gene variants will be cloned and prepared in recombinant form for further characterization.

Název v anglickém jazyce

Comparison of cystatins of four species of helmints

Popis výsledku anglicky

Cystatins, the inhibitors of cysteine peptidases, are expressed and released by many parasitic organisms. They are participating on regulation of biological processes, e.g., the modification of the host immune response. Our study is primarily focused oncharacterization of molecular, biochemical and immunological properties of cystatins of four model species representing the four main groups of helmints: Fascioloides magna (Trematoda), Hymenolepis diminuta (Cestoda), Diplozoon paradoxum (Monogenea) andTrichinella spiralis (Nematoda). The excretory-secretory products were isolated, purified and fractionated and the identification of cystatins is in process. The RNA was isolated, reverse transcription performed and prepared cDNA used in PCR with specific primers designed for amplification of particular cystatine genes. Obtained amplicons will be sequenced and the selected cystatine gene variants will be cloned and prepared in recombinant form for further characterization.

Druh

O - Ostatní výsledky

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - Evropské centrum ichtyoparazitologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů