Cystatins of helminths: characterization of molecules with immunomodulatory potential

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088207" target="_blank" >RIV/00216224:14310/16:00088207 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cystatins of helminths: characterization of molecules with immunomodulatory potential

Popis výsledku v původním jazyce

Cystatins ­ cysteine peptidase inhibitors are produced by a wide range of organisms, including parasites, where they act as modulators of the host immune system. In the frame of our research we focused on characterization of cystatins produced by four helminth species representing most important groups of helminth parasites, namely Eudiplozoon nipponicum (Monogenea), Fascioloides magna (Trematoda), Hymenolepis diminuta (Cestoda) and Trichinella spiralis (Nematoda). We performed a bacterial expression of optimized cystatin genes in recombinant forms (E. coli BL21 cells, pET32a and/or pET19b plasmid vector). The recombinant cystatins were purified via His­tag using metal affinity chromatography (Ni­NTA) and successful production of cystatin was confirmed by mass spectrometric methods (MALDI TOF/TOF).

Název v anglickém jazyce

Cystatins of helminths: characterization of molecules with immunomodulatory potential

Popis výsledku anglicky

Cystatins ­ cysteine peptidase inhibitors are produced by a wide range of organisms, including parasites, where they act as modulators of the host immune system. In the frame of our research we focused on characterization of cystatins produced by four helminth species representing most important groups of helminth parasites, namely Eudiplozoon nipponicum (Monogenea), Fascioloides magna (Trematoda), Hymenolepis diminuta (Cestoda) and Trichinella spiralis (Nematoda). We performed a bacterial expression of optimized cystatin genes in recombinant forms (E. coli BL21 cells, pET32a and/or pET19b plasmid vector). The recombinant cystatins were purified via His­tag using metal affinity chromatography (Ni­NTA) and successful production of cystatin was confirmed by mass spectrometric methods (MALDI TOF/TOF).

Druh

O - Ostatní výsledky

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů