The endocrine disruptive potential of phytoplankton exudates.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00067014" target="_blank" >RIV/00216224:14310/13:00067014 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The endocrine disruptive potential of phytoplankton exudates.
Popis výsledku v původním jazyce
This study focus on endocrine disrupting potential of metabolites with high environmental relevance which phytoplankton excrete into the surrounding environment during normal physiological processes (exudates). The novel embryo model with transgenic zebrafish strain (cyp19a1b-GFP) was chosen to assess the in vivo estrogenicity. Green fluorescent protein (GFP) is expresses under the control of aromatase B (cyp19a1b) promoter which is induced by estrogens. Phenotypic toxicity and neurotoxicity was also assessed in zebrafish embryos. Battery of in vitro methods focused on diverse endocrine disruption modes of action ? estrogenic, anti/androgenic, glucocorticoid and anti/retinoid was used to investigate potential effects on receptor level. Concentrations of a set of prioritised phytoestrogens were determined analytically by HPLC-MSMS to investigate to which extent they could be responsible for the observed biological effects.
Název v anglickém jazyce
The endocrine disruptive potential of phytoplankton exudates.
Popis výsledku anglicky
This study focus on endocrine disrupting potential of metabolites with high environmental relevance which phytoplankton excrete into the surrounding environment during normal physiological processes (exudates). The novel embryo model with transgenic zebrafish strain (cyp19a1b-GFP) was chosen to assess the in vivo estrogenicity. Green fluorescent protein (GFP) is expresses under the control of aromatase B (cyp19a1b) promoter which is induced by estrogens. Phenotypic toxicity and neurotoxicity was also assessed in zebrafish embryos. Battery of in vitro methods focused on diverse endocrine disruption modes of action ? estrogenic, anti/androgenic, glucocorticoid and anti/retinoid was used to investigate potential effects on receptor level. Concentrations of a set of prioritised phytoestrogens were determined analytically by HPLC-MSMS to investigate to which extent they could be responsible for the observed biological effects.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
DJ - Znečištění a kontrola vody
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů