Analysis of estrogenicity, toxicity and neurotoxicity of Microcystis aerigunosa, Cylidrospermopsis raciborskii and Scenedesmus quadricauda exudates with zebrafish (Danio rerio) embryos.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00067013" target="_blank" >RIV/00216224:14310/12:00067013 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of estrogenicity, toxicity and neurotoxicity of Microcystis aerigunosa, Cylidrospermopsis raciborskii and Scenedesmus quadricauda exudates with zebrafish (Danio rerio) embryos.

Popis výsledku v původním jazyce

Cyanobacteria are known to synthesize mixture of diverse biologically active compounds which are partially excreted into natural water bodies. The structural identity as well as potential biological activity and toxicological properties of many of thesecompounds are still unknown. In this study the zebrafish embryo model was used to investigate the potential estrogenicity, neurotoxicity and general toxicity of exudates from laboratory cultures of two cyanobacteria species Microcystis aerigunosa and Cylidrospermopsis raciborskii and one green algae Scenedesmus quadricauda. Exudate samples were collected after 21-day exponential growth separating the liquid phase from cells; after that the liquid phase was extracted by solid phase extraction. A transgenic zebrafish strain (cyp19a1b-GFP, Brion et al., PloS ONE 2012, e36069) was used to assess the estrogenicity. This strain expresses green fluorescent protein under the control of aromatase B (cyp19a1b) promoter, which is induced by estrog

Název v anglickém jazyce

Analysis of estrogenicity, toxicity and neurotoxicity of Microcystis aerigunosa, Cylidrospermopsis raciborskii and Scenedesmus quadricauda exudates with zebrafish (Danio rerio) embryos.

Popis výsledku anglicky

Cyanobacteria are known to synthesize mixture of diverse biologically active compounds which are partially excreted into natural water bodies. The structural identity as well as potential biological activity and toxicological properties of many of thesecompounds are still unknown. In this study the zebrafish embryo model was used to investigate the potential estrogenicity, neurotoxicity and general toxicity of exudates from laboratory cultures of two cyanobacteria species Microcystis aerigunosa and Cylidrospermopsis raciborskii and one green algae Scenedesmus quadricauda. Exudate samples were collected after 21-day exponential growth separating the liquid phase from cells; after that the liquid phase was extracted by solid phase extraction. A transgenic zebrafish strain (cyp19a1b-GFP, Brion et al., PloS ONE 2012, e36069) was used to assess the estrogenicity. This strain expresses green fluorescent protein under the control of aromatase B (cyp19a1b) promoter, which is induced by estrog

Druh

O - Ostatní výsledky

CEP obor

DJ - Znečištění a kontrola vody

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů