Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00067588" target="_blank" >RIV/00216224:14310/13:00067588 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol8/80100100.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol8/80100100.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores

Popis výsledku v původním jazyce

Cheap, rapid and user friendly tools for detection of microorganisms were intensively discussed during last decades. The use of immunospecific biosensors seems advantageous for samples originating from clinical, environmental and agricultural area, foodprocessing and military. The piezoelectric (quartz crystal microbalance, QCM) and optical (surface plasmon resonance, SPR) biosensors represented by QCM Analyzer and Biacore 3000 were compared for future development of real-time and label-free microbialbiosensors. The spores of Bacillus atrophaeus were used as a non-pathogenic surrogate for B. anthracis. Seven commercially available antibodies were immobilised on the sensor surfaces to select the most promising one. Various immobilisation techniques were used. The total time of analysis did not exceed 30 min and the estimated limits of detection (LOD) were 10^6 CFU mL-1 and 10^5 CFU mL-1 in case of QCM and SPR, respectively.

Název v anglickém jazyce

Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores

Popis výsledku anglicky

Cheap, rapid and user friendly tools for detection of microorganisms were intensively discussed during last decades. The use of immunospecific biosensors seems advantageous for samples originating from clinical, environmental and agricultural area, foodprocessing and military. The piezoelectric (quartz crystal microbalance, QCM) and optical (surface plasmon resonance, SPR) biosensors represented by QCM Analyzer and Biacore 3000 were compared for future development of real-time and label-free microbialbiosensors. The spores of Bacillus atrophaeus were used as a non-pathogenic surrogate for B. anthracis. Seven commercially available antibodies were immobilised on the sensor surfaces to select the most promising one. Various immobilisation techniques were used. The total time of analysis did not exceed 30 min and the estimated limits of detection (LOD) were 10^6 CFU mL-1 and 10^5 CFU mL-1 in case of QCM and SPR, respectively.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

13

Strana od-do

100-112

Kód UT WoS článku

000316562800008

EID výsledku v databázi Scopus

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