Piezoelectric biosensors for detection of microorganisms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00075645" target="_blank" >RIV/00216224:14740/14:00075645 - isvavai.cz</a>

Výsledek na webu

<a href="http://web2.mendelu.cz/af_239_nanotech/data/soubory/sbornik2014.pdf#page=34" target="_blank" >http://web2.mendelu.cz/af_239_nanotech/data/soubory/sbornik2014.pdf#page=34</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Piezoelectric biosensors for detection of microorganisms

Popis výsledku v původním jazyce

Piezoelectric biosensors provide fast, specific and economic way of detection for a wide range of analytes ranging from metabolites to microorganisms. Specific antibodies were immobilised on gold electrodes of piezoelectric crystal and the biosensor wasapplied for real-time detection of model microorganisms. Three nonpathogenic strains of Escherichia coli (BL21, DH5 alpha and K-12) were used because of easy cultivation and availability of antibodies. Several methods of antibody immobilisation were compared. The immobilisation of reduced antibody using Sulfo-SMCC was the most effective achieving the limit of detection (LOD) 10^5 CFU x mL-1. Active and passive operation modes of quartz crystal microbalance were compared concluding that for detection ofmicroorganisms, both approaches are practically identical. The developed biosensor was connected to the cyclone air sampler allowing detection of microorganisms disseminated in the form of aerosol inside bioaerosol chamber. The achieved L

Název v anglickém jazyce

Piezoelectric biosensors for detection of microorganisms

Popis výsledku anglicky

Piezoelectric biosensors provide fast, specific and economic way of detection for a wide range of analytes ranging from metabolites to microorganisms. Specific antibodies were immobilised on gold electrodes of piezoelectric crystal and the biosensor wasapplied for real-time detection of model microorganisms. Three nonpathogenic strains of Escherichia coli (BL21, DH5 alpha and K-12) were used because of easy cultivation and availability of antibodies. Several methods of antibody immobilisation were compared. The immobilisation of reduced antibody using Sulfo-SMCC was the most effective achieving the limit of detection (LOD) 10^5 CFU x mL-1. Active and passive operation modes of quartz crystal microbalance were compared concluding that for detection ofmicroorganisms, both approaches are practically identical. The developed biosensor was connected to the cyclone air sampler allowing detection of microorganisms disseminated in the form of aerosol inside bioaerosol chamber. The achieved L

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků

ISBN

9788021068421

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

32-35

Název nakladatele

Masarykova univerzita

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

3. 6. 2014

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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