Investigation of interaction between magnetic silica particles and lambda phage DNA fragment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00072703" target="_blank" >RIV/00216224:14310/13:00072703 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/13:00213505 RIV/00216305:26620/13:PU105678

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jpba.2013.07.039" target="_blank" >http://dx.doi.org/10.1016/j.jpba.2013.07.039</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jpba.2013.07.039" target="_blank" >10.1016/j.jpba.2013.07.039</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Investigation of interaction between magnetic silica particles and lambda phage DNA fragment

Popis výsledku v původním jazyce

Nucleic acids belong to the most important molecules and therefore the understanding of their properties, function and behavior is crucial. Even though a range of analytical and biochemical methods have been developed for this purpose, one common step isessential for all of them isolation of the nucleic acid from the from complex sample matrix. The use of magnetic particles for the separation of nucleic acids has many advantages over other isolation methods. In this study, an isolation procedure for extraction of DNA was optimized. Each step of the isolation process including washing, immobilization and elution was optimized and therefore the efficiency was increased from 1.7% to 28.7% and the total time was shortened from 75 to 30 min comparing to the previously described method. Quantification of the particular parameter influence was performed by square-wave voltammetry using hanging drop mercury electrode.

Název v anglickém jazyce

Investigation of interaction between magnetic silica particles and lambda phage DNA fragment

Popis výsledku anglicky

Nucleic acids belong to the most important molecules and therefore the understanding of their properties, function and behavior is crucial. Even though a range of analytical and biochemical methods have been developed for this purpose, one common step isessential for all of them isolation of the nucleic acid from the from complex sample matrix. The use of magnetic particles for the separation of nucleic acids has many advantages over other isolation methods. In this study, an isolation procedure for extraction of DNA was optimized. Each step of the isolation process including washing, immobilization and elution was optimized and therefore the efficiency was increased from 1.7% to 28.7% and the total time was shortened from 75 to 30 min comparing to the previously described method. Quantification of the particular parameter influence was performed by square-wave voltammetry using hanging drop mercury electrode.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Pharmaceutical and Biomedical Analysis

ISSN

0731-7085

e-ISSN

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Svazek periodika

86

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

65-72

Kód UT WoS článku

000325671200007

EID výsledku v databázi Scopus

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