Next-generation sequencing and genome characterization of red clover and its wild relative zig-zag clover

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00073258" target="_blank" >RIV/00216224:14310/14:00073258 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Next-generation sequencing and genome characterization of red clover and its wild relative zig-zag clover

Popis výsledku v původním jazyce

The genus Trifolium is classified taxonomically into the agronomically outstanding Fabaceae family. It comprises species known as clovers, both wild and cultivated. Recently, interspecific hybridization of tetraploid red clover with related wild octoploid zig-zag clover (T. medium) has been used for the introgression of useful traits into red clover. De novo assembly of the T. pratense genome after Illumina sequencing was achieved. The current assembly comprises ~314.6 Mbp of draft nucleotide sequence.Overall, 47398 protein coding genes were annotated from 64761 predicted genes. Resistance genes, genes for leghemoglobins and nodule-specific cystein-rich peptides were identified. Sequencing data of T. medium were obtained with average genome coverage 20.7x (x = 3.5 Gbp). Species-specific SNP calling for coding sequences was performed and over 924837 SNPs between parental genotypes used for hybridization were discovered. Species-specific DNA markers for T. pratense (23299) and T.

Název v anglickém jazyce

Next-generation sequencing and genome characterization of red clover and its wild relative zig-zag clover

Popis výsledku anglicky

The genus Trifolium is classified taxonomically into the agronomically outstanding Fabaceae family. It comprises species known as clovers, both wild and cultivated. Recently, interspecific hybridization of tetraploid red clover with related wild octoploid zig-zag clover (T. medium) has been used for the introgression of useful traits into red clover. De novo assembly of the T. pratense genome after Illumina sequencing was achieved. The current assembly comprises ~314.6 Mbp of draft nucleotide sequence.Overall, 47398 protein coding genes were annotated from 64761 predicted genes. Resistance genes, genes for leghemoglobins and nodule-specific cystein-rich peptides were identified. Sequencing data of T. medium were obtained with average genome coverage 20.7x (x = 3.5 Gbp). Species-specific SNP calling for coding sequences was performed and over 924837 SNPs between parental genotypes used for hybridization were discovered. Species-specific DNA markers for T. pratense (23299) and T.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

International Conference on ENHANCED GENEPOOL UTILIZATION - Capturing wild relative and landrace diversity for crop improvement, Cambridge, United Kingdom, 16-20 June 2014. Book of Abstracts.

ISBN

9789290439950

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

91-92

Název nakladatele

Bioversity International, Rome, Italy

Místo vydání

United Kingdom

Místo konání akce

Cambridge, UK

Datum konání akce

1. 1. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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